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PMID: 1976638 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Epithelial integrin alpha 6 beta 4: complete primary structure of alpha 6 and variant forms of beta 4.

The Journal of cell biology ·Vol. 111 ·No. 4 ·1990-10-00 ·Pages 1593-604

Tamura RN, Rozzo C, Starr L, Chambers J, Reichardt LF, Cooper HM, Quaranta V

Abstract

The integrin alpha 6 beta 4 is a heterodimer predominantly expressed by epithelia. While no definite receptor function has yet been assigned to it, this integrin may mediate adhesive and/or migratory functions of epithelial cells. We have determined the complete primary structure of both the alpha 6 and beta 4 subunits from cDNA clones isolated from pancreatic carcinoma cell line libraries. The deduced amino acid sequence of alpha 6 is homologous to other integrin alpha chains (18-26% identity). Antibodies to an alpha 6 carboxy terminus peptide immunoprecipitated alpha 6 beta 4 complexes from carcinoma cells and alpha 6 beta 1 complexes from platelets, providing further evidence for the association of alpha 6 with more than one beta subunit. The deduced amino acid sequence of beta 4 predicts an extracellular portion homologous to other integrin beta chains, and a unique cytoplasmic domain comprised of greater than 1,000 residues. This agrees with the structures of the beta 4 cDNAs from normal epithelial cells (Suzuki, S., and Y. Naitoh. 1990. EMBO [Eur. Mol. Biol. Organ.] J. 9:757-763; Hogervost, F., I. Kuikman, A. E. G. Kr. von dem Borne, and A. Sonnenberg. 1990. EMBO [Eur. Mol. Biol. Organ.] J. 9:765-770). Compared to these structures, however, the beta 4 cDNAs that we have cloned from carcinoma cells contain extra sequences. One of these is located in the 5'-untranslated region, and may encode regulatory sequences. Another specifies a segment of 70 amino acids in the cytoplasmic tail. Amplification by reverse transcription-polymerase chain reaction of mRNA indicated that multiple forms of beta 4 may exist, possibly due to cell-type specific alternative splicing. The unique structure of beta 4 suggests its involvement in novel cytoskeletal interactions. Consistent with this possibility, alpha 6 beta 4 is mostly concentrated on the basal surface of epithelial cells, but does not colocalize with components of adhesion plaques.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Compartmentation Cloning, Molecular Fluorescent Antibody Technique Humans Integrins/chemistry,genetics Macromolecular Substances Molecular Sequence Data Poly A/analysis Polymerase Chain Reaction RNA, Messenger/analysis Sequence Alignment Sequence Homology, Nucleic Acid
Chemicals
Integrins Macromolecular Substances RNA, Messenger Poly A
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Tamura R N
Department of Immunology, IMM-8, Research Institute of Scripps Clinic, La Jolla, California 92037.
Rozzo C
Starr L
Chambers J
Reichardt L F
Cooper H M
Quaranta V
References (42)
42 references, click to expand
  1. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  2. Efficient isolation of genes by using antibody probes.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
  3. Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1585-8 PMID: 3856838
  4. Identification of human glucocorticoid receptor complementary DNA clones by epitope selection.
    Science. 1985 May 10;228(4700):740-2 PMID: 2581314
  5. Six monoclonal antibodies to human pancreatic cancer antigens.
    Cancer Res. 1987 Mar 1;47(5):1367-76 PMID: 3815343
  6. Integrins: a family of cell surface receptors.
    Cell. 1987 Feb 27;48(4):549-54 PMID: 3028640
  7. Structure of the platelet membrane glycoprotein IIb. Homology to the alpha subunits of the vitronectin and fibronectin membrane receptors.
    J Biol Chem. 1987 Jun 25;262(18):8476-82 PMID: 2439501
  8. A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody.
    J Biol Chem. 1987 Jul 25;262(21):10376-83 PMID: 3301835
  9. Amino acid sequence of the vitronectin receptor alpha subunit and comparative expression of adhesion receptor mRNAs.
    J Biol Chem. 1987 Oct 15;262(29):14080-5 PMID: 2443500
  10. Amino acid sequence of the human fibronectin receptor.
    J Cell Biol. 1987 Sep;105(3):1183-90 PMID: 2958481
  11. New perspectives in cell adhesion: RGD and integrins.
    Science. 1987 Oct 23;238(4826):491-7 PMID: 2821619
  12. Cell surface receptors for extracellular matrix molecules.
    Annu Rev Cell Biol. 1987;3:179-205 PMID: 2825736
  13. The Drosophila PS2 antigen is an invertebrate integrin that, like the fibronectin receptor, becomes localized to muscle attachments.
    Cell. 1987 Dec 24;51(6):929-40 PMID: 2961459
  14. Comparison of cDNA-derived protein sequences of the human fibronectin and vitronectin receptor alpha-subunits and platelet glycoprotein IIb.
    Biochemistry. 1987 Dec 15;26(25):8158-65 PMID: 2450560
  15. cDNA cloning and complete primary structure of the alpha subunit of a leukocyte adhesion glycoprotein, p150,95.
    EMBO J. 1987 Dec 20;6(13):4023-8 PMID: 3327687
  16. Amino acid sequence of the alpha subunit of human leukocyte adhesion receptor Mo1 (complement receptor type 3).
    J Cell Biol. 1988 Jun;106(6):2153-8 PMID: 2454931
  17. Amino acid sequence of the murine Mac-1 alpha chain reveals homology with the integrin family and an additional domain related to von Willebrand factor.
    EMBO J. 1988 May;7(5):1371-8 PMID: 3044779
  18. The human leukocyte adhesion glycoprotein Mac-1 (complement receptor type 3, CD11b) alpha subunit. Cloning, primary structure, and relation to the integrins, von Willebrand factor and factor B.
    J Biol Chem. 1988 Sep 5;263(25):12403-11 PMID: 2457584
  19. Laminin receptor on platelets is the integrin VLA-6.
    Nature. 1988 Dec 1;336(6198):487-9 PMID: 2973567
  20. Identification of a murine Peyer's patch--specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4 alpha.
    Cell. 1989 Jan 13;56(1):37-46 PMID: 2463092
  21. Primary structure of the leukocyte function-associated molecule-1 alpha subunit: an integrin with an embedded domain defining a protein superfamily.
    J Cell Biol. 1989 Feb;108(2):703-12 PMID: 2537322
  22. A novel vitronectin receptor integrin (alpha v beta x) is responsible for distinct adhesive properties of carcinoma cells.
    Cell. 1989 Apr 7;57(1):59-69 PMID: 2467745
  23. Association of the VLA alpha 6 subunit with a novel protein. A possible alternative to the common VLA beta 1 subunit on certain cell lines.
    J Biol Chem. 1989 Apr 15;264(11):6529-35 PMID: 2649503
  24. A novel integrin (alpha E beta 4) from human epithelial cells suggests a fourth family of integrin adhesion receptors.
    EMBO J. 1989 Mar;8(3):673-80 PMID: 2542022
  25. The primary structure of the VLA-2/collagen receptor alpha 2 subunit (platelet GPIa): homology to other integrins and the presence of a possible collagen-binding domain.
    J Cell Biol. 1989 Jul;109(1):397-407 PMID: 2545729
  26. An alternative cytoplasmic domain of the integrin beta 3 subunit.
    Proc Natl Acad Sci U S A. 1989 Jul;86(14):5415-8 PMID: 2787511
  27. The primary structure of the alpha 4 subunit of VLA-4: homology to other integrins and a possible cell-cell adhesion function.
    EMBO J. 1989 May;8(5):1361-8 PMID: 2788572
  28. Analysis of the tumor-associated antigen TSP-180. Identity with alpha 6-beta 4 in the integrin superfamily.
    J Biol Chem. 1989 Sep 15;264(26):15515-21 PMID: 2475502
  29. Developmentally regulated alternative splicing of Drosophila integrin PS2 alpha transcripts.
    Cell. 1989 Oct 6;59(1):185-95 PMID: 2507168
  30. A novel integrin beta subunit is associated with the vitronectin receptor alpha subunit (alpha v) in a human osteosarcoma cell line and is a substrate for protein kinase C.
    EMBO J. 1989 Oct;8(10):2955-65 PMID: 2479539
  31. Alternative splicing in the control of gene expression.
    Annu Rev Genet. 1989;23:527-77 PMID: 2694943
  32. Amino acid sequence of a novel integrin beta 4 subunit and primary expression of the mRNA in epithelial cells.
    EMBO J. 1990 Mar;9(3):757-63 PMID: 2311577
  33. Cloning and sequence analysis of beta-4 cDNA: an integrin subunit that contains a unique 118 kd cytoplasmic domain.
    EMBO J. 1990 Mar;9(3):765-70 PMID: 2311578
  34. Characterization through cDNA cloning of galactoprotein b3 (Gap b3), a cell surface membrane glycoprotein showing enhanced expression on oncogenic transformation. Identification of Gap b3 as a member of the integrin superfamily.
    J Biol Chem. 1990 Apr 25;265(12):7016-21 PMID: 1691184
  35. Complete amino acid sequence of a novel integrin beta subunit (beta 6) identified in epithelial cells using the polymerase chain reaction.
    J Biol Chem. 1990 Jul 15;265(20):11502-7 PMID: 2365683
  36. Integrins and tumor cell dissemination.
    Cancer Cells. 1989 Dec;1(4):119-26 PMID: 2701367
  37. Methods for assessing immunologic and biologic properties of iodinated peptide hormones.
    Methods Enzymol. 1975;37:223-33 PMID: 1128244
  38. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line.
    Cancer Res. 1976 Feb;36(2 Pt 1):467-75 PMID: 1260746
  39. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  40. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  41. Calcium-binding proteins and the molecular basis of calcium action.
    Int Rev Cytol. 1982;77:1-61 PMID: 6757171
  42. Cell-substratum interaction of cultured avian osteoclasts is mediated by specific adhesion structures.
    J Cell Biol. 1984 Nov;99(5):1696-705 PMID: 6436255
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.4.1593
Published
1990-10-00
Pages
1593-604
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116232
Subset
IM
Grants
NCI NIH HHS · CA 47541 · United States
NCI NIH HHS · CA 47858 · United States
NCI NIH HHS · CA 50286 · United States
Databases
GENBANK
X53586, X53587
External Links
PubMed source DOI: 10.1083/jcb.111.4.1593 PMC Full Text View journal details
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