DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

Nucleic acids research ·Vol. 18 ·No. 22 ·1990-11-25 ·Pages 6531-5

Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV

Abstract

Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross. Here we describe a new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. We suggest that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.

MeSH Terms

Base Composition Base Sequence Crossing Over, Genetic DNA/biosynthesis,chemistry Genetic Markers Humans Molecular Sequence Data Neurospora crassa/genetics Nucleotide Mapping Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Soybeans/genetics Zea mays/genetics

Chemicals

Genetic Markers DNA

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Williams J G

Central Research and Development Department, E.I. du Pont de Nemours & Co., Inc, Wilmington, DE 19880.

Kubelik A R

Livak K J

Rafalski J A

Tingey S V

References (7)

7 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/18.22.6531

Published

1990-11-25

Pages

6531-5

Language

English

Region

England

NLM ID

0411011

PMCID

PMC332606

Subset

External Links

PubMed source DOI: 10.1093/nar/18.22.6531 PMC Full Text View journal details

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DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

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