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PMID: 19820940 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Dse1 may control cross talk between the pheromone and filamentation pathways in yeast.

Current genetics ·Vol. 55 ·No. 6 ·2009-12-00 ·Pages 611-21

Draper E, Dubrovskyi O, Bar EE, Stone DE

Abstract

The filamentous/invasive growth pathway is activated by nutrient limitation in the haploid form of the yeast Saccharomyces cerevisiae, whereas exposure to mating-pheromone causes cells to differentiate into gametes. Although these two pathways respond to very different stimuli and generate very different responses, they utilize many of the same signaling components. This implies the need for robust mechanisms to maintain signal fidelity. Dse1 was identified in an allele-specific suppressor screen for proteins that interact with the pheromone-responsive Gbetagamma, and found to bind both to a Gbetagamma-affinity column, and to the shared MEKK, Ste11. Although overexpression of Dse1 stimulated invasive growth and transcription of both filamentation and mating-specific transcriptional reporters, deletion of DSE1 had no effect on these outputs. In contrast, pheromone hyper-induced transcription of the filamentation reporter in cells lacking Dse1 and in cells expressing a mutant form of Gbeta that exhibits diminished interaction with Dse1. Thus, the interaction of Dse1 with both Gbeta and Ste11 may be designed to control cross talk between the pheromone and filamentation pathways.

MeSH Terms
Binding Sites Carrier Proteins/genetics,physiology Cell Cycle/drug effects Chromatography, Affinity GTP-Binding Protein beta Subunits/genetics,physiology Gene Knockdown Techniques Genes, Reporter Haploidy MAP Kinase Kinase Kinases/genetics,physiology MAP Kinase Signaling System/drug effects,genetics,physiology Protein Interaction Mapping Protein Precursors/pharmacology Recombinant Fusion Proteins/physiology Saccharomyces cerevisiae/cytology,genetics,growth & development,physiology Saccharomyces cerevisiae Proteins/genetics,pharmacology,physiology Signal Transduction/drug effects,genetics,physiology Two-Hybrid System Techniques
Chemicals
Carrier Proteins DSE1 protein, S cerevisiae GTP-Binding Protein beta Subunits MF(ALPHA)1 protein, S cerevisiae Protein Precursors Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Ste4 protein, S cerevisiae MAP Kinase Kinase Kinases Ste11 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Draper Edward
Laboratory for Molecular Biology (M/C 567), Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607, USA.
Dubrovskyi Oleksii
Bar Eli E
Stone David E
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
1432-0983
DOI
10.1007/s00294-009-0274-6
Published
2009-12-00
Epub
2009-00-10
Pages
611-21
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/s00294-009-0274-6 View journal details
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