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PMID: 1985198 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Evolution of human immunodeficiency virus type 1 nef and long terminal repeat sequences over 4 years in vivo and in vitro.

Journal of virology ·Vol. 65 ·No. 1 ·1991-01-00 ·Pages 225-31

Delassus S, Cheynier R, Wain-Hobson S

Abstract

The evolution of an 851-bp segment of the human immunodeficiency virus type 1 (HIV-1) genome encoding the nef open reading frame and U3/R elements of the long terminal repeat has been followed over a 4-year period in vivo and in vitro. The population of viral sequences at any given time was established by sequencing cloned polymerase chain reaction products. The samples studied were derived from the same man for whom a detailed analysis of the tat gene was previously described (A. Meyerhans, R. Cheynier, J. Albert, M. Seth, S. Kwok, J. Sninsky, L. Morfeldt-Manson, B. Asjö, and S. Wain-Hobson, Cell 58:901-910, 1989). Once again in vitro culture resulted in the selection of minor forms. Over a 4-year period in vivo, there was no obvious selection for, or outgrowth of, any particular nef or U3/R sequence. Few defective nef protein sequences were observed, which argues against nef acting as a negative regulatory factor. Although no functionally defective promoter/trans-activation-responsive elements were identified, the transactivation efficiencies varied between 0.2 and 2 times that of the control. The sequence encoding the most efficient trans-activation-responsive region did not outgrow others. The extreme genetic heterogeneity of the different samples of the locus, either in vivo or in vitro, indicates that there is no such thing as a single, distinct HIV sequence. It is suggested that different HIV-1 loci evolve independently, recombination being responsible for their uncoupling.

MeSH Terms
Base Sequence Biological Evolution Cell Line Cloning, Molecular Genes, Viral Genetic Vectors HIV Long Terminal Repeat HIV Seropositivity HIV-1/genetics,isolation & purification Humans Male Molecular Sequence Data Oligonucleotide Probes Open Reading Frames Polymerase Chain Reaction Promoter Regions, Genetic Sequence Homology, Nucleic Acid Time Factors Transfection
Chemicals
Oligonucleotide Probes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Delassus S
Laboratoire de Rétrovirologie Moléculaire, Institut Pasteur, Paris, France.
Cheynier R
Wain-Hobson S
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.1.225-231.1991
Published
1991-01-00
Pages
225-31
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240509
Subset
IM
Databases
GENBANK
M58193, M58194, M58195, M58196, M58197, M58198, M58199, M58200, M58201, M58202, M58203, M58204, M58205, M58206, M58207, M58208, M58209, M58210, M58211, M58212, M58213, M58214, M58215, M58216, M58217, M58218, M58219, M58220, M58221, M58222
External Links
PubMed source DOI: 10.1128/JVI.65.1.225-231.1991 PMC Full Text View journal details
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