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PMID: 1986251 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Induction of growth arrest by a temperature-sensitive p53 mutant is correlated with increased nuclear localization and decreased stability of the protein.

Molecular and cellular biology ·Vol. 11 ·No. 1 ·1991-01-00 ·Pages 582-5

Ginsberg D, Michael-Michalovitz D, Ginsberg D, Oren M

Abstract

A temperature-sensitive mutant of p53, p53Val-135, was found to be able to arrest cell proliferation when overexpressed at 32.5 degrees C. While much of the protein was cytoplasmic in cells proliferating at 37.5 degrees C, it became predominantly nuclear at 32.5 degrees C. Concomitantly, p53Val-135 became destabilized, although not to the extent seen in primary fibroblasts.

MeSH Terms
Animals Cell Compartmentation Cell Division Cell Nucleus/metabolism Cell Transformation, Neoplastic/pathology Fluorescent Antibody Technique In Vitro Techniques Mice Mutation Temperature Tumor Suppressor Protein p53/physiology
Chemicals
Tumor Suppressor Protein p53
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ginsberg D
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Michael-Michalovitz D
Ginsberg D
Oren M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.1.582-585.1991
Published
1991-01-00
Pages
582-5
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359675
Subset
IM
Grants
NCI NIH HHS · R01 CA 40099 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.1.582-585.1991 PMC Full Text View journal details
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