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PMID: 2851728 Published · ppublish English Journal Article

Characterization of mutant p53-hsp72/73 protein-protein complexes by transient expression in monkey COS cells.

Molecular and cellular biology ·Vol. 8 ·No. 9 ·1988-09-00 ·Pages 3740-7

Stürzbecher HW, Addison C, Jenkins JR

Abstract

Several mutant, but not wild-type, p53 proteins form complexes with hsp72/73 heat shock-related proteins in simian virus 40-transformed monkey COS cells. We carried out a detailed biochemical and structural mapping analysis of p53 and report here that p53-hsp72/73 complex formation showed considerable structural specificity. Such complexes were remarkably stable, but unlike analogous complexes formed between p53 and simian virus 40 T antigen, they did not form in in vitro association assays. p53-hsp72/73 complex formation in vivo appears to be dependent on aspects of mutant p53 protein conformation. However, absence of the conformation-sensitive epitope recognized by monoclonal antibody PAb 246 was not reliably diagnostic of such complexes, nor was p53-hsp72173 binding reliably diagnostic of oncogenic activation.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic Chromosome Deletion DNA Transposable Elements Genes Heat-Shock Proteins/genetics,metabolism Mutation Neoplasm Proteins/genetics,metabolism Nuclear Proteins/genetics Phosphoproteins/genetics,metabolism Plasmids Protein Biosynthesis Simian virus 40/genetics Transcription, Genetic Tumor Suppressor Protein p53
Chemicals
DNA Transposable Elements Heat-Shock Proteins Neoplasm Proteins Nuclear Proteins Phosphoproteins Tumor Suppressor Protein p53
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stürzbecher H W
Cell Proliferation Laboratory, Marie Curie Research Institute, Oxted, Surrey, England.
Addison C
Jenkins J R
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35 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.9.3740-3747.1988
Published
1988-09-00
Pages
3740-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365431
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.9.3740-3747.1988 PMC Full Text View journal details
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