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PMID: 1986252 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Heat shock-induced interactions of heat shock transcription factor and the human hsp70 promoter examined by in vivo footprinting.

Molecular and cellular biology ·Vol. 11 ·No. 1 ·1991-01-00 ·Pages 586-92

Abravaya K, Phillips B, Morimoto RI

Abstract

Genomic footprinting of the human hsp70 promoter reveals that heat shock induces a rapid binding of a factor, presumably heat shock transcription factor, to a region encompassing five contiguous NGAAN sequences, three perfect and two imperfect matches to the consensus sequence. Arrays of inverted NGAAN sequences have been defined as the heat shock element. No protein is bound to the heat shock element prior to or after recovery from heat shock. Heat shock does not perturb the binding of factors to other regulatory elements in the promoter which contribute to basal expression of the hsp70 gene.

MeSH Terms
Base Sequence Binding Sites DNA-Binding Proteins/metabolism Gene Expression Regulation HeLa Cells Heat-Shock Proteins/genetics,metabolism Hot Temperature Humans Methylation Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Sulfuric Acid Esters/chemistry Transcription Factors/metabolism
Chemicals
DNA-Binding Proteins Heat-Shock Proteins Sulfuric Acid Esters Transcription Factors dimethyl sulfate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Abravaya K
Department of Biochemistry, Molecular Biology, and Cellular Biology, Northwestern University, Evanston, Illinois 60208.
Phillips B
Morimoto R I
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30 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.1.586-592.1991
Published
1991-01-00
Pages
586-92
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359677
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.11.1.586-592.1991 PMC Full Text View journal details
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