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PMID: 3146692 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Key features of heat shock regulatory elements.

Molecular and cellular biology ·Vol. 8 ·No. 9 ·1988-09-00 ·Pages 3761-9

Amin J, Ananthan J, Voellmy R

Abstract

The promoters of heat shock protein genes are among the best-studied inducible eucaryotic promoters. Regions responsible for heat regulation have been identified previously by deletion experiments with several different heat shock genes. In this paper the critical importance of two novel features of heat shock regulatory elements was investigated. First, the elements were modular and, as a consequence, displayed a characteristic 5-nucleotide periodicity produced by multiple GAA blocks that were arranged in alternating orientations and at 2-nucleotide intervals. Functional heat shock regulatory elements appeared to include three or more of these blocks. Second, the nucleotides at the two positions immediately upstream from GAA segments played an important role in defining the competence of regulatory elements.

MeSH Terms
Animals Base Sequence Drosophila melanogaster/genetics Genes Genes, Regulator Heat-Shock Proteins/genetics Hot Temperature Humans Molecular Sequence Data Plasmids Regulatory Sequences, Nucleic Acid Species Specificity
Chemicals
Heat-Shock Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Amin J
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101.
Ananthan J
Voellmy R
References (46)
46 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.9.3761-3769.1988
Published
1988-09-00
Pages
3761-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365434
Subset
IM
Grants
NIGMS NIH HHS · GM-31125 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.9.3761-3769.1988 PMC Full Text View journal details
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