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PMID: 6252541 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two genes for the major heat-shock protein of Drosophila melanogaster arranged as an inverted repeat.

Nucleic acids research ·Vol. 8 ·No. 2 ·1980-01-25 ·Pages 235-52

Goldschmidt-Clermont M

Abstract

The physical maps of three cloned D. melanogaster DNA fragments with genes for the 70,000 dalton heat-shock protein (hsp 70) are presented. Fragment 122 contains two genes in diverging orientation, forming an inverted repeat around a central spacer. The other two fragments, which are found as polymorphic variants in the fly population, have related structures; they differ by the deletion or the insertion of large DNA segments. The sequence homologies between 122 and a plasmid with two hsp 70 genes in tandem repetition was investigated by heteroduplex analysis. In addition to the basic gene units, the segments share other homologous sequence elements which are found in different combinations near the beginning of the genes.

MeSH Terms
Animals Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Drosophila melanogaster/metabolism Genes Hot Temperature Microscopy, Electron Molecular Weight Nucleic Acids Plasmids Protein Biosynthesis Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Recombinant Nucleic Acids DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Goldschmidt-Clermont M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.2.235
Published
1980-01-25
Pages
235-52
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327264
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.2.235 PMC Full Text View journal details
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