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PMID: 1987146 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of Escherichia coli katE, which encodes catalase HPII.

Journal of bacteriology ·Vol. 173 ·No. 2 ·1991-01-00 ·Pages 514-20

von Ossowski I, Mulvey MR, Leco PA, Borys A, Loewen PC

Abstract

A 3,466-bp nucleotide sequence containing the katE gene of Escherichia coli has been determined. An open reading frame of 2,259 bp was found and was preceded by a potential ribosome-binding site. The predicted N-terminal sequence agreed with the sequence determined by direct amino acid sequencing, and the predicted direction of transcription was confirmed by expression of the gene cloned in both directions behind a T7 promoter. The start site of transcription was determined to be 127 bp upstream from the start of the open reading frame, and a potential RNA polymerase-binding site similar to a sequence preceding the xthA gene, which is also controlled by the KatF protein, was identified. The predicted sequence of the 753-amino-acid protein was compared with known sequences of other catalases, revealing significant similarity to the shorter catalases, including the residues in the putative active site and residues involved in heme binding.

Related Genes
katE xthA
MeSH Terms
Amino Acid Sequence Animals Base Sequence Catalase/genetics Escherichia coli/enzymology,genetics Genes, Bacterial Humans Isoenzymes/genetics Molecular Sequence Data Oligonucleotide Probes Plasmids Rats Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Isoenzymes Oligonucleotide Probes Catalase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
von Ossowski I
Department of Microbiology, University of Manitoba, Winnipeg, Canada.
Mulvey M R
Leco P A
Borys A
Loewen P C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.2.514-520.1991
Published
1991-01-00
Pages
514-20
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207040
Subset
IM
Databases
GENBANK
M55161, M64121, M64122, M64123, M64124, M64125, M64126, M64127, M64128, M64129
External Links
PubMed source DOI: 10.1128/jb.173.2.514-520.1991 PMC Full Text View journal details
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