IMPROVED METHODS FOR THE PREPARATION OF THE SPECIFIC POLYSACCHARIDES OF PNEUMOCOCCUS.
The specific polysaccharides of Types I and V pneumococcus give sharp equivalence zones and show maximal precipitation with homologous rabbit antisera only when carefully prepared from cultures which have not been neutralized with alkali. The precipitating power of S V toward homologous rabbit antiserum falls off in 0.1 N NaOH even more rapidly than that of S I, dropping to 7 per cent of the original value in 6 days at room temperature. The alkaline solution develops a large absorption peak at 270 mmicro. Directions are given for the preparation of S VII and S XII.
10 references, click to expand
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CHEMOIMMUNOLOGICAL STUDIES ON THE SOLUBLE SPECIFIC SUBSTANCE OF PNEUMOCOCCUS : I. THE ISOLATION AND PROPERTIES OF THE ACETYL POLYSACCHARIDE OF PNEUMOCOCCUS TYPE I.J Exp Med. 1933 Nov 30;58(6):731-55 PMID: 19870226
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A Partially Defined Medium for Cultivation of Pneumococcus.J Bacteriol. 1945 Apr;49(4):401-9 PMID: 16560933
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Measurement and preservation of antibodies in human sera.J Immunol. 1949 Feb;61(2):153-9 PMID: 18113743
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ANTIBODY FORMATION IN VOLUNTEERS FOLLOWING INJECTION OF PNEUMOCOCCI OR THEIR TYPE-SPECIFIC POLYSACCHARIDES.J Exp Med. 1946 Mar 31;83(4):303-20 PMID: 19871532
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A colorimetric microanalytical method for acetate and fluoroacetate.J Biol Chem. 1949 Feb;177(2):571-5 PMID: 18110435
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THE SPECIFIC POLYSACCHARIDES OF TYPES I, II, AND III PNEUMOCOCCUS : A REVISION OF METHODS AND DATA.J Exp Med. 1936 Sep 30;64(4):559-72 PMID: 19870553
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THE PRECIPITIN REACTION BETWEEN TYPE III PNEUMOCOCCUS POLYSACCHARIDE AND HOMOLOGOUS ANTIBODY : III. A QUANTITATIVE STUDY AND A THEORY OF THE REACTION MECHANISM.J Exp Med. 1935 Mar 31;61(4):563-91 PMID: 19870380
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A steam distillation apparatus suitable for micro-Kjeldahl analysis.Biochem J. 1942 Dec;36(10-12):790-1 PMID: 16747508
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THE PRECIPITIN REACTION BETWEEN TYPE III PNEUMOCOCCUS POLYSACCHARIDE AND HOMOLOGOUS ANTIBODY : II. CONDITIONS FOR QUANTITATIVE PRECIPITATION OF ANTIBODY IN HORSE SERA.J Exp Med. 1935 Mar 31;61(4):559-62 PMID: 19870379
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ANTIBODY FORMATION IN MEN FOLLOWING INJECTION OF FOUR TYPE-SPECIFIC POLYSACCHARIDES OF PNEUMOCOCCUS.J Exp Med. 1947 Feb 28;85(3):227-30 PMID: 19871610
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