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PMID: 19920355 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Th22 cells represent a distinct human T cell subset involved in epidermal immunity and remodeling.

The Journal of clinical investigation ·Vol. 119 ·No. 12 ·2009-12-00 ·Pages 3573-85

Eyerich S, Eyerich K, Pennino D, Carbone T, Nasorri F, Pallotta S, Cianfarani F, Odorisio T, Traidl-Hoffmann C, Behrendt H, Durham SR, Schmidt-Weber CB, Cavani A

Abstract

Th subsets are defined according to their production of lineage-indicating cytokines and functions. In this study, we have identified a subset of human Th cells that infiltrates the epidermis in individuals with inflammatory skin disorders and is characterized by the secretion of IL-22 and TNF-alpha, but not IFN-gamma, IL-4, or IL-17. In analogy to the Th17 subset, cells with this cytokine profile have been named the Th22 subset. Th22 clones derived from patients with psoriasis were stable in culture and exhibited a transcriptome profile clearly separate from those of Th1, Th2, and Th17 cells; it included genes encoding proteins involved in tissue remodeling, such as FGFs, and chemokines involved in angiogenesis and fibrosis. Primary human keratinocytes exposed to Th22 supernatants expressed a transcriptome response profile that included genes involved in innate immune pathways and the induction and modulation of adaptive immunity. These proinflammatory Th22 responses were synergistically dependent on IL-22 and TNF-alpha. Furthermore, Th22 supernatants enhanced wound healing in an in vitro injury model, which was exclusively dependent on IL-22. In conclusion, the human Th22 subset may represent a separate T cell subset with a distinct identity with respect to gene expression and function, present within the epidermal layer in inflammatory skin diseases. Future strategies directed against the Th22 subset may be of value in chronic inflammatory skin disorders.

MeSH Terms
Adult Clone Cells Dermatitis/genetics,immunology,pathology Epidermal Cells Epidermis/immunology Gene Expression Profiling Humans Immunity, Innate In Vitro Techniques Interferon-gamma/metabolism Interleukin-17/metabolism Interleukin-4/metabolism Interleukins/genetics,metabolism Keratinocytes/immunology Psoriasis/genetics,immunology,pathology Receptors, CCR10/metabolism T-Lymphocyte Subsets/cytology,immunology T-Lymphocytes, Helper-Inducer/cytology,immunology Tumor Necrosis Factor-alpha/metabolism Wound Healing/genetics,immunology
Chemicals
CCR10 protein, human IL4 protein, human Interleukin-17 Interleukins Receptors, CCR10 Tumor Necrosis Factor-alpha Interleukin-4 Interferon-gamma interleukin-22
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Eyerich Stefanie
Molecular Immunology, Department of Allergy and Clinical Immunology, National Heart and Lung Institute, Imperial College, London, United Kingdom.
Eyerich Kilian
Pennino Davide
Carbone Teresa
Nasorri Francesca
Pallotta Sabatino
Cianfarani Francesca
Odorisio Teresa
Traidl-Hoffmann Claudia
Behrendt Heidrun
Durham Stephen R
Schmidt-Weber Carsten B
Cavani Andrea
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
1558-8238
DOI
10.1172/JCI40202
Published
2009-12-00
Epub
2009-00-16
Pages
3573-85
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC2786807
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI40202 PMC Full Text View journal details
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