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PMID: 1995949 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cellular factors regulate transactivation of human immunodeficiency virus type 1.

Journal of virology ·Vol. 65 ·No. 3 ·1991-03-00 ·Pages 1392-9

Barry PA, Pratt-Lowe E, Unger RE, Luciw PA

Abstract

It is hypothesized that the immediate-early (IE) gene products of human cytomegalovirus (CMV) and the transactivator (TAT) of human immunodeficiency virus type 1 (HIV-1) regulate HIV-1 gene expression through mechanisms involving host cell factors. By using transient transfection assays with the gene for chloramphenicol acetyltransferase (CAT) under the transcriptional control of the HIV-1 long terminal repeat (LTR), we examined transactivation of the LTR by plasmids that express either the HIV-1 gene for TAT or human CMV IE. The ratio of the level of transactivation by CMV IE to the level of transactivation by TAT varied up to 1,000-fold between cell types. The difference in the activities of these transactivators in various cell types was not a consequence of differential expression of the transactivator gene. Analysis of RNA species initiated in the HIV-1 LTR supports the conclusion that cellular factors regulate the level of elongation of the transcription complex on the LTR. Furthermore, evidence that in some cell types the predominant mechanism of transactivation by HIV-1 TAT involves posttranscriptional processes is presented.

MeSH Terms
Animals Cell Line Chloramphenicol O-Acetyltransferase/genetics,metabolism Gene Expression Regulation, Viral Gene Products, tat/genetics HIV Long Terminal Repeat HIV-1/genetics Humans Recombinant Fusion Proteins/metabolism Transcription, Genetic Transcriptional Activation Transfection tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, tat Recombinant Fusion Proteins tat Gene Products, Human Immunodeficiency Virus Chloramphenicol O-Acetyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Barry P A
Department of Medical Pathology, University of California, Davis 95616.
Pratt-Lowe E
Unger R E
Luciw P A
References (53)
53 references, click to expand
  1. An inducible transcription factor activates expression of human immunodeficiency virus in T cells.
    Nature. 1987 Apr 16-22;326(6114):711-3 PMID: 3031512
  2. Molecular interactions of cytomegalovirus and the human and simian immunodeficiency viruses.
    J Med Primatol. 1990;19(3-4):327-37 PMID: 2172542
  3. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  4. Identification and characterization of the human cytomegalovirus immediate-early region 2 gene that stimulates gene expression from an inducible promoter.
    J Virol. 1987 Oct;61(10):3214-21 PMID: 3041043
  5. Alterations in binding characteristics of the human immunodeficiency virus enhancer factor.
    J Virol. 1988 Jan;62(1):218-25 PMID: 3257103
  6. Anti-termination of transcription within the long terminal repeat of HIV-1 by tat gene product.
    Nature. 1987 Dec 3-9;330(6147):489-93 PMID: 2825027
  7. Immediate-early gene region of human cytomegalovirus trans-activates the promoter of human immunodeficiency virus.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8642-6 PMID: 2825201
  8. Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus.
    EMBO J. 1987 Dec 1;6(12):3761-70 PMID: 3428273
  9. Differences in cell-type-specific blocks to immediate early gene expression and DNA replication of human, simian and murine cytomegalovirus.
    J Gen Virol. 1988 Feb;69 ( Pt 2):355-74 PMID: 2828515
  10. trans-activation and autoregulation of gene expression by the immediate-early region 2 gene products of human cytomegalovirus.
    J Virol. 1988 Apr;62(4):1167-79 PMID: 2831379
  11. Transcriptional but not translational regulation of HIV-1 by the tat gene product.
    Nature. 1988 Apr 7;332(6164):551-3 PMID: 2833703
  12. HIV and HCMV coinfect brain cells in patients with AIDS.
    Virology. 1988 Jul;165(1):286-90 PMID: 2838967
  13. Purification of the human immunodeficiency virus type 1 enhancer and TAR binding proteins EBP-1 and UBP-1.
    EMBO J. 1988 Jul;7(7):2117-30 PMID: 3138113
  14. Mutational analysis of the 5' non-coding region of human immunodeficiency virus type 1: effects of secondary structure on translation.
    EMBO J. 1988 Sep;7(9):2831-7 PMID: 3181141
  15. Structural arrangements of transcription control domains within the 5'-untranslated leader regions of the HIV-1 and HIV-2 promoters.
    Genes Dev. 1988 Sep;2(9):1101-14 PMID: 2847959
  16. Human cytomegalovirus ie1 transactivates the alpha promoter-enhancer via an 18-base-pair repeat element.
    J Virol. 1989 Mar;63(3):1435-40 PMID: 2536844
  17. Structure, sequence, and position of the stem-loop in tar determine transcriptional elongation by tat through the HIV-1 long terminal repeat.
    Genes Dev. 1989 Apr;3(4):547-58 PMID: 2470647
  18. Regulated expression of early and late RNAs and proteins from the human cytomegalovirus immediate-early gene region.
    J Virol. 1989 Jun;63(6):2699-708 PMID: 2542583
  19. Functional characterization of a complex protein-DNA-binding domain located within the human immunodeficiency virus type 1 long terminal repeat leader region.
    J Virol. 1989 Aug;63(8):3213-9 PMID: 2545899
  20. HIV-1 TAT "activates" presynthesized RNA in the nucleus.
    Cell. 1989 Jul 28;58(2):269-79 PMID: 2546679
  21. Expression of the acidic nuclear immediate-early protein (IE1) of human cytomegalovirus in stable cell lines and its preferential association with metaphase chromosomes.
    Virology. 1989 Oct;172(2):584-600 PMID: 2477948
  22. HIV-1 Tat protein increases transcriptional initiation and stabilizes elongation.
    Cell. 1989 Oct 20;59(2):283-92 PMID: 2553266
  23. Human chromosome 12 is required for elevated HIV-1 expression in human-hamster hybrid cells.
    Science. 1989 Oct 27;246(4929):488-91 PMID: 2683071
  24. Regulation of human T cell leukemia virus expression.
    FASEB J. 1990 Feb 1;4(2):169-75 PMID: 2404818
  25. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  26. Production of mouse mammary tumor virus by cultured cells in the absence and presence of hormones: assay by molecular hybridization.
    Virology. 1975 May;65(1):135-47 PMID: 49975
  27. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma.
    Int J Cancer. 1977 May 15;19(5):621-6 PMID: 68013
  28. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 PMID: 286319
  29. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  30. High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.
    Nucleic Acids Res. 1984 Jul 25;12(14):5707-17 PMID: 6589587
  31. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  32. The location of cis-acting regulatory sequences in the human T cell lymphotropic virus type III (HTLV-III/LAV) long terminal repeat.
    Cell. 1985 Jul;41(3):813-23 PMID: 2988790
  33. Multiple spliced and unspliced transcripts from human cytomegalovirus immediate-early region 2 and evidence for a common initiation site within immediate-early region 1.
    J Virol. 1985 Dec;56(3):665-75 PMID: 2999423
  34. Post-transcriptional regulation accounts for the trans-activation of the human T-lymphotropic virus type III.
    Nature. 1986 Feb 13-19;319(6054):555-9 PMID: 3003584
  35. The trans-activator gene of the human T cell lymphotropic virus type III is required for replication.
    Cell. 1986 Mar 28;44(6):941-7 PMID: 2420471
  36. The trans-activator gene of HTLV-III is essential for virus replication.
    Nature. 1986 Mar 27-Apr 2;320(6060):367-71 PMID: 3007995
  37. Activation of the AIDS retrovirus promoter by the cellular transcription factor, Sp1.
    Science. 1986 May 9;232(4751):755-9 PMID: 3008338
  38. Trans-activation of human immunodeficiency virus occurs via a bimodal mechanism.
    Cell. 1986 Sep 26;46(7):973-82 PMID: 3530501
  39. Expression and characterization of the trans-activator of HTLV-III/LAV virus.
    Science. 1986 Nov 21;234(4779):988-92 PMID: 3490693
  40. Elevated levels of mRNA can account for the trans-activation of human immunodeficiency virus.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9734-8 PMID: 3025848
  41. Trans-activation of the human immunodeficiency virus long terminal repeat sequence by DNA viruses.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9759-63 PMID: 2432602
  42. Regulation of mRNA accumulation by a human immunodeficiency virus trans-activator protein.
    Cell. 1987 Feb 27;48(4):691-701 PMID: 3643816
  43. Adenovirus E1A protein paradigm viral transactivator.
    Annu Rev Genet. 1989;23:141-61 PMID: 2533472
  44. Promoter-specific trans activation and repression by human cytomegalovirus immediate-early proteins involves common and unique protein domains.
    J Virol. 1990 Apr;64(4):1556-65 PMID: 2157043
  45. Transacting transcriptional activation of human spumaretrovirus LTR in infected cells.
    Virology. 1990 Apr;175(2):568-71 PMID: 2158186
  46. Cytomegalovirus activates transcription directed by the long terminal repeat of human immunodeficiency virus type 1.
    J Virol. 1990 Jun;64(6):2932-40 PMID: 2159554
  47. A cDNA for a protein that interacts with the human immunodeficiency virus Tat transactivator.
    Science. 1990 Jun 29;248(4963):1650-3 PMID: 2194290
  48. Simian foamy virus type 1 is a retrovirus which encodes a transcriptional transactivator.
    J Virol. 1990 Aug;64(8):3598-604 PMID: 2370676
  49. Human chromosome 12 encodes a species-specific factor which increases human immunodeficiency virus type 1 tat-mediated trans activation in rodent cells.
    J Virol. 1990 Sep;64(9):4565-7 PMID: 2200890
  50. TAR-independent activation of the HIV-1 LTR: evidence that tat requires specific regions of the promoter.
    Cell. 1990 Aug 24;62(4):757-67 PMID: 2201451
  51. Trans-activation by HIV-1 Tat via a heterologous RNA binding protein.
    Cell. 1990 Aug 24;62(4):769-76 PMID: 2117500
  52. A nuclear translational block imposed by the HIV-1 U3 region is relieved by the Tat-TAR interaction.
    Cell. 1990 Sep 21;62(6):1123-33 PMID: 2205397
  53. A rapid, sensitive, and inexpensive assay for chloramphenicol acetyltransferase.
    DNA. 1987 Apr;6(2):173-8 PMID: 3472867
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.3.1392-1399.1991
Published
1991-03-00
Pages
1392-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC239917
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.65.3.1392-1399.1991 PMC Full Text View journal details
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