Home LiteratureArticle Details
PMID: 1997426 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cell and receptor requirements for streptococcal pyrogenic exotoxin T-cell mitogenicity.

Infection and immunity ·Vol. 59 ·No. 3 ·1991-03-00 ·Pages 1210-4

Leonard BA, Lee PK, Jenkins MK, Schlievert PM

Abstract

Streptococcal pyrogenic exotoxins (SPEs) A, B, and C, like other members of the pyrogenic toxin family, are able to cause toxic shock-like syndromes. One of the major properties of these toxins is the ability to induce T-cell proliferation. Characterization of T cell mitogenicity associated with SPEs A, B, and C was undertaken. SPEs A, B, and C were mitogenic for C57BL10/SnJ and BALB/cWAT T cells, with activities differing in intensity depending on the mouse strain and toxin employed. SPE-induced, T-cell-proliferative activity was dependent on class II major histocompatibility complex molecules expressed on antigen-presenting cells. The abilities of SPEs A, B, and C to preferentially stimulate murine cells with certain T-cell receptor V beta s were investigated by fluorescence-activated cell sorter analysis. SPE A preferentially activated T cells expressing V beta 8 but not V beta 3, 6, or 11, while SPEs B and C preferentially stimulated T cells which did not express any of the tested V beta s.

MeSH Terms
Animals Antibodies, Monoclonal Antigen-Presenting Cells/drug effects,immunology Bacterial Proteins Exotoxins/metabolism,toxicity Histocompatibility Antigens Class II/biosynthesis Lymphocyte Activation/drug effects Membrane Proteins Mice Mice, Inbred BALB C Mice, Inbred C57BL Mitogens/metabolism,toxicity Receptors, Antigen, T-Cell/drug effects T-Lymphocytes/drug effects,immunology
Chemicals
Antibodies, Monoclonal Bacterial Proteins Exotoxins Histocompatibility Antigens Class II Membrane Proteins Mitogens Receptors, Antigen, T-Cell SpeA protein, Streptococcus pyogenes erythrogenic toxin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Leonard B A
Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455.
Lee P K
Jenkins M K
Schlievert P M
References (39)
39 references, click to expand
  1. Nonspecific T-lymphocyte mitogenesis by pyrogenic exotoxins from group A streptococci and Staphylococcus aureus.
    Infect Immun. 1979 Sep;25(3):1075-7 PMID: 387590
  2. Nonspecific and specific immunological mitogenicity by group A streptococcal pyrogenic exotoxins.
    Infect Immun. 1978 Dec;22(3):681-8 PMID: 365764
  3. Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxic-shock syndrome.
    J Infect Dis. 1981 Apr;143(4):509-16 PMID: 6972418
  4. Characterization of the human cellular immune response to purified group A streptococcal blastogen A1.
    J Immunol. 1982 Apr;128(4):1631-6 PMID: 7037952
  5. Toxicity of staphylococcal toxic shock syndrome toxin 1 in rabbits.
    Infect Immun. 1984 Nov;46(2):314-7 PMID: 6500691
  6. Toxic-shock-syndrome toxin 1-induced proliferation of lymphocytes: comparison of the mitogenic response of human, murine, and rabbit lymphocytes.
    J Infect Dis. 1985 Jan;151(1):65-72 PMID: 3871225
  7. Expression of the cloned toxic shock syndrome toxin 1 gene (tst) in vivo with a rabbit uterine model.
    Infect Immun. 1985 Oct;50(1):304-9 PMID: 4044040
  8. The nucleotide and partial amino acid sequence of toxic shock syndrome toxin-1.
    J Biol Chem. 1986 Nov 25;261(33):15783-6 PMID: 3782090
  9. A rabbit model of toxic shock syndrome that uses a constant, subcutaneous infusion of toxic shock syndrome toxin 1.
    Infect Immun. 1987 May;55(5):1070-6 PMID: 3570455
  10. Clinical and bacteriologic observations of a toxic shock-like syndrome due to Streptococcus pyogenes.
    N Engl J Med. 1987 Jul 16;317(3):146-9 PMID: 3299086
  11. Streptococcal outbreaks and erythrogenic toxin type A.
    Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Aug;266(1-2):104-15 PMID: 3122461
  12. Nonpurulent response to toxic shock syndrome toxin 1-producing Staphylococcus aureus. Relationship to toxin-stimulated production of tumor necrosis factor.
    J Immunol. 1988 Feb 1;140(3):949-53 PMID: 3339245
  13. Toxic shock syndrome toxin-1 induces inositol phospholipid turnover, protein kinase C translocation, and calcium mobilization in human T cells.
    J Immunol. 1988 Feb 15;140(4):1250-5 PMID: 2830336
  14. Self-tolerance eliminates T cells specific for Mls-modified products of the major histocompatibility complex.
    Nature. 1988 Mar 3;332(6159):35-40 PMID: 3126396
  15. T-cell receptor V beta use predicts reactivity and tolerance to Mlsa-encoded antigens.
    Nature. 1988 Mar 3;332(6159):40-5 PMID: 3126397
  16. Binding of staphylococcal enterotoxin A to accessory cells is a requirement for its ability to activate human T cells.
    J Immunol. 1988 Apr 15;140(8):2484-8 PMID: 3258609
  17. T cell stimulation by staphylococcal enterotoxins. Clonally variable response and requirement for major histocompatibility complex class II molecules on accessory or target cells.
    J Exp Med. 1988 May 1;167(5):1697-707 PMID: 3259256
  18. The T-cell repertoire is heavily influenced by tolerance to polymorphic self-antigens.
    Nature. 1988 Oct 27;335(6193):796-801 PMID: 3263572
  19. Toxic shock syndrome-associated staphylococcal and streptococcal pyrogenic toxins are potent inducers of tumor necrosis factor production.
    Infect Immun. 1989 Jan;57(1):291-4 PMID: 2642470
  20. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice.
    Cell. 1989 Jan 13;56(1):27-35 PMID: 2521300
  21. T-cell responses to Mls and to bacterial proteins that mimic its behavior.
    Immunol Rev. 1989 Feb;107:61-88 PMID: 2522086
  22. The MHC molecule I-E is necessary but not sufficient for the clonal deletion of V beta 11-bearing T cells.
    J Exp Med. 1989 Apr 1;169(4):1405-19 PMID: 2538552
  23. High-affinity binding of staphylococcal enterotoxins A and B to HLA-DR.
    Nature. 1989 May 18;339(6221):221-3 PMID: 2785644
  24. Toxic shock syndrome toxin 1 binds to major histocompatibility complex class II molecules.
    Proc Natl Acad Sci U S A. 1989 Jun;86(11):4210-4 PMID: 2542966
  25. V beta-specific stimulation of human T cells by staphylococcal toxins.
    Science. 1989 May 19;244(4906):811-3 PMID: 2524876
  26. Class II MHC molecules are specific receptors for staphylococcus enterotoxin A.
    Science. 1989 May 19;244(4906):817-20 PMID: 2658055
  27. Severe group A streptococcal infections associated with a toxic shock-like syndrome and scarlet fever toxin A.
    N Engl J Med. 1989 Jul 6;321(1):1-7 PMID: 2659990
  28. Quantification and toxicity of group A streptococcal pyrogenic exotoxins in an animal model of toxic shock syndrome-like illness.
    J Clin Microbiol. 1989 Aug;27(8):1890-2 PMID: 2504778
  29. Bacterial toxins as probes for the T-cell antigen receptor.
    Immunol Today. 1989 Aug;10(8):262-4 PMID: 2803504
  30. The toxicity of staphylococcal enterotoxin B in mice is mediated by T cells.
    J Exp Med. 1990 Feb 1;171(2):455-64 PMID: 2303780
  31. Molecular requirements for T cell activation by the staphylococcal toxic shock syndrome toxin-1.
    J Immunol. 1990 Mar 15;144(6):2089-95 PMID: 2313089
  32. The staphylococcal enterotoxins and their relatives.
    Science. 1990 May 11;248(4956):705-11 PMID: 2185544
  33. Nucleotide sequence of the streptococcal pyrogenic exotoxin type B gene and relationship between the toxin and the streptococcal proteinase precursor.
    J Bacteriol. 1990 Aug;172(8):4536-42 PMID: 2198264
  34. Activation of murine T cells by streptococcal pyrogenic exotoxin type A. Requirement for MHC class II molecules on accessory cells and identification of V beta elements in T cell receptor of toxin-reactive T cells.
    J Immunol. 1990 Nov 15;145(10):3170-6 PMID: 2230113
  35. Separation of mouse spleen cells by passage through columns of sephadex G-10.
    J Immunol Methods. 1974 Aug;5(3):239-47 PMID: 4610054
  36. Evidence for cell-receptor activity in lymphocyte stimulation by staphylococcal enterotoxin.
    J Immunol. 1975 Jul;115(1):49-53 PMID: 1080166
  37. Purification and characterization of group A streptococcal pyrogenic exotoxin type C.
    Infect Immun. 1977 May;16(2):673-9 PMID: 324918
  38. Biological effects of staphylococcal enterotoxin A on human peripheral lymphocytes.
    Infect Immun. 1978 Oct;22(1):62-8 PMID: 730351
  39. A new staphylococcal enterotoxin, enterotoxin F, associated with toxic-shock-syndrome Staphylococcus aureus isolates.
    Lancet. 1981 May 9;1(8228):1017-21 PMID: 6112412
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.3.1210-1214.1991
Published
1991-03-00
Pages
1210-4
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258393
Subset
IM
Grants
NIAID NIH HHS · AI27998 · United States
NIAID NIH HHS · AI28635 · United States
NHLBI NIH HHS · HL36611 · United States
External Links
PubMed source DOI: 10.1128/iai.59.3.1210-1214.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2