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PMID: 2000398 Published · ppublish English Comparative Study Journal Article

Angiotensin II induces secretion of plasminogen activator inhibitor 1 and a tissue metalloprotease inhibitor-related protein from rat brain astrocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 5 ·1991-03-01 ·Pages 1928-32

Olson JA, Shiverick KT, Ogilvie S, Buhi WC, Raizada MK

Abstract

The present study investigates angiotensin (Ang) II effects on secretory protein synthesis in brain astrocytes cultured from neonatal and 21-day-old rats. Ang II-induced changes in the de novo synthesis of [35S]methionine-labeled secretory proteins were visualized using two-dimensional NaDodSO4/PAGE. Astrocytes from 21-day-old rat brain possess specific high-affinity receptors for Ang II. These cells express two Ang II-induced secretory proteins with Mr 55,000 (AISP-55K) and Mr 30,000 (AISP-30K), which were time- and dose-dependent (EC50, 1 nM). [Sar1, Ile8]Ang II (where Sar is sarcosine) inhibited Ang II-induced secretion of AISP-55K but not AISP-30K. N-terminal amino acid sequencing indicates that AISP-55K is identical to rat plasminogen activator inhibitor 1, whereas AISP-30K exhibits 72-81% identity to three closely related proteins: human tissue inhibitor of metalloproteases, a rat phorbol ester-induced protein, and the murine growth-responsive protein 16C8. Immunofluorescent staining with rat plasminogen activator inhibitor 1 antibody was induced in the majority of cells in culture after Ang II treatment of astrocytes from 21-day-old rat brains. Absence of this response to Ang II in astrocytes from neonatal rat brain provides evidence that this action of Ang II on astrocytes is developmentally regulated.

MeSH Terms
Aging Amino Acid Sequence Angiotensin II/pharmacology Animals Animals, Newborn Astrocytes/drug effects,physiology Brain/growth & development,physiology Cells, Cultured Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Glycoproteins/biosynthesis Kinetics Methionine/metabolism Molecular Sequence Data Molecular Weight Nerve Tissue Proteins/biosynthesis,isolation & purification Plasminogen Inactivators/metabolism Rats Sequence Homology, Nucleic Acid Sulfur Radioisotopes Tissue Inhibitor of Metalloproteinases
Chemicals
Glycoproteins Nerve Tissue Proteins Plasminogen Inactivators Sulfur Radioisotopes Tissue Inhibitor of Metalloproteinases Angiotensin II Methionine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Olson J A
Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville 32610.
Shiverick K T
Ogilvie S
Buhi W C
Raizada M K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.5.1928
Published
1991-03-01
Pages
1928-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51139
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.5.1928 PMC Full Text View journal details
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