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PMID: 3024122 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A growth-responsive gene (16C8) in normal mouse fibroblasts homologous to a human collagenase inhibitor with erythroid-potentiating activity: evidence for inducible and constitutive transcripts.

Nucleic acids research ·Vol. 14 ·No. 22 ·1986-11-25 ·Pages 8863-78

Edwards DR, Waterhouse P, Holman ML, Denhardt DT

Abstract

We present the DNA sequence of an essentially full-length cDNA clone of 16C8, a growth factor-inducible gene isolated from a mouse embryo fibroblast cDNA library. The 0.9-kb mRNA encodes an Mr 22,500 protein that has substantial homology to a human protein with the reported abilities to potentiate erythroid differentiation and to inhibit collagenases and other tissue metalloproteinases. The N-terminus of the predicted protein has a hydrophobic nature characteristic of secreted proteins, and two potential sites for N-linked glycosylation are present. The cytoplasmic concentration of 16C8 mRNA is maximal in mid G1 at about 6 h after serum stimulation of quiescent fibroblasts. Northern blot analysis showed a progressive reduction in the size of the induced 16C8 transcripts with increasing time after serum stimulation. This was shown to be due to the reduction in length of the poly(A) tails. S1 analysis of the 5' portion of the mRNA revealed the presence of three different species of transcript, only one of which was inducible.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cells, Cultured Cloning, Molecular DNA/analysis Enzyme Inhibitors/genetics Fibroblasts/metabolism Genes/drug effects Growth Substances/pharmacology Humans Mice Microbial Collagenase/antagonists & inhibitors Nucleic Acid Hybridization Plasmids Protein Biosynthesis Transcription, Genetic
Chemicals
Enzyme Inhibitors Growth Substances DNA Microbial Collagenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Edwards D R
Waterhouse P
Holman M L
Denhardt D T
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.22.8863
Published
1986-11-25
Pages
8863-78
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311916
Subset
IM
Databases
GENBANK
X04684
External Links
PubMed source DOI: 10.1093/nar/14.22.8863 PMC Full Text View journal details
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