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PMID: 3837843 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcriptional regulation of two serum-induced RNAs in mouse fibroblasts: equivalence of one species to B2 repetitive elements.

Molecular and cellular biology ·Vol. 5 ·No. 11 ·1985-11-00 ·Pages 3280-8

Edwards DR, Parfett CL, Denhardt DT

Abstract

We obtained eight cDNA clones that define five genes whose expression (appearance of transcripts in the cytoplasm) is enhanced when quiescent mouse fibroblasts are stimulated with serum to divide. Two of these clones (designated 49C8 and 16C8) correspond to RNA species that are present in the cytoplasm of quiescent cells at very low levels. After serum stimulation, the level of 16C8 mRNA rose more rapidly than that of 49C8 RNA, reaching a maximum around 6 to 12 h. The data suggest that 49C8 and 16C8 RNAs are induced as a result of independent stimuli. Either fibroblast growth factor or 12-tetradecanoylphorbol-13-acetate alone could induce 16C8 expression almost as effectively as serum; in contrast, 49C8 was not efficiently induced by epidermal growth factor, fibroblast growth factor, insulin, or 12-tetradecanoylphorbol-13-acetate. Inhibitors of transcription and translation diminished the induction of 16C8, while 49C8 expression was sensitive to actinomycin D but not cycloheximide or 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole. In vitro transcription experiments with isolated nuclei revealed a peak in transcriptional activity of the 16C8 gene at around 3 h after serum stimulation. Sequence analysis of the 49C8 cDNA clone showed greater than 90% homology of a large portion to a consensus rodent B2 repetitive element.

MeSH Terms
Animals Base Sequence Blood Cattle Cells, Cultured Culture Media Cycloheximide/pharmacology Dactinomycin/pharmacology Dichlororibofuranosylbenzimidazole/pharmacology Embryo, Mammalian Fibroblasts/metabolism Mice Nucleic Acid Hybridization Polyribosomes/metabolism Protein Biosynthesis/drug effects Protein Processing, Post-Translational/drug effects RNA Processing, Post-Transcriptional/drug effects RNA, Messenger/genetics Transcription, Genetic/drug effects
Chemicals
Culture Media RNA, Messenger Dactinomycin Dichlororibofuranosylbenzimidazole Cycloheximide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Edwards D R
Parfett C L
Denhardt D T
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38 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.11.3280-3288.1985
Published
1985-11-00
Pages
3280-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369145
Subset
IM
Databases
GENBANK
M11944
External Links
PubMed source DOI: 10.1128/mcb.5.11.3280-3288.1985 PMC Full Text View journal details
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