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PMID: 2012180 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interleukin-1 beta and tumor necrosis factor-alpha induce gene expression and production of leukocyte chemotactic factors, colony-stimulating factors, and interleukin-6 in human mesangial cells.

The American journal of pathology ·Vol. 138 ·No. 4 ·1991-04-00 ·Pages 991-1003

Zoja C, Wang JM, Bettoni S, Sironi M, Renzi D, Chiaffarino F, Abboud HE, Van Damme J, Mantovani A, Remuzzi G

Abstract

The capacity of human cultured mesangial cells to produce soluble factors potentially relevant for mechanisms of inflammation and immunity at the glomerular site was analyzed. The nature of the secreted factors initially was investigated by Northern blot analysis using total cellular RNAs isolated from resting and activated mesangial cells. On exposure of mesangial cells to human recombinant interleukin-1 beta (IL-1 beta), high levels of interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1) mRNAs were detected. Similar transcripts were found after stimulation with human recombinant tumor necrosis factor-alpha (TNF-alpha). Active secretion of IL-8 was documented by radioimmunoassay in supernatants of mesangial cells activated by either IL-1 beta or TNF-alpha. Using an in vitro migration assay, supernatants from resting mesangial cells were found to be devoid of any chemotactic activity for granulocytes or monocytes. On stimulation with IL-1 beta, however, mesangial cell supernatants expressed MCP-1 biologic activity detected as induction of a strong migratory response for human monocytes but not for granulocytes. In addition, IL-1 beta and TNF-alpha induced high levels of granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF) mRNAs. Similarly IL-1 beta and TNF-alpha induced the interleukin-6 (IL-6) gene and active secretion of its mature protein. These data strongly support an effector role for mesangial cells in modulating immune-inflammatory responses in glomeruli. Release of cytokines may activate not only infiltrating inflammatory cells through short paracrine pathways, but also mesangial cells themselves through an autocrine pathway.

MeSH Terms
Cells, Cultured Chemokine CCL2 Chemotactic Factors/biosynthesis,genetics Chemotaxis, Leukocyte Colony-Stimulating Factors/biosynthesis,genetics Cytokines/pharmacology Gene Expression Regulation/drug effects Glomerular Mesangium/cytology,metabolism,physiology Humans Interleukin-1/pharmacology Interleukin-6/biosynthesis,genetics Interleukin-8/genetics Tumor Necrosis Factor-alpha/pharmacology
Chemicals
Chemokine CCL2 Chemotactic Factors Colony-Stimulating Factors Cytokines Interleukin-1 Interleukin-6 Interleukin-8 Tumor Necrosis Factor-alpha
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Zoja C
Mario Negri Institute for Pharmacological Research, Bergamo, Italy.
Wang J M
Bettoni S
Sironi M
Renzi D
Chiaffarino F
Abboud H E
Van Damme J
Mantovani A
Remuzzi G
References (56)
56 references, click to expand
  1. Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1).
    Science. 1985 Oct 18;230(4723):291-6 PMID: 2996129
  2. Glomerular cell culture.
    Lab Invest. 1985 Aug;53(2):122-31 PMID: 3894792
  3. Cell culture approaches to the analysis of glomerular inflammation.
    Kidney Int. 1986 Aug;30(2):246-54 PMID: 3531678
  4. Tumor necrosis factor type alpha stimulates human endothelial cells to produce granulocyte/macrophage colony-stimulating factor.
    Proc Natl Acad Sci U S A. 1986 Oct;83(19):7467-71 PMID: 3489939
  5. Contractile effects of TxA2 and endoperoxide analogues on cultured rat glomerular mesangial cells.
    Am J Physiol. 1986 Dec;251(6 Pt 2):F1029-35 PMID: 3538903
  6. Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9679-83 PMID: 2948184
  7. Tumor necrosis factor is chemotactic for monocytes and polymorphonuclear leukocytes.
    J Immunol. 1987 Mar 1;138(5):1469-74 PMID: 3805724
  8. Interleukin-HP1, a T cell-derived hybridoma growth factor that supports the in vitro growth of murine plasmacytomas.
    J Exp Med. 1987 Mar 1;165(3):641-9 PMID: 3493321
  9. Tumor necrosis factor and interleukin 1: cytokines with multiple overlapping biological activities.
    Lab Invest. 1987 Mar;56(3):234-48 PMID: 3029503
  10. Human CSF-1: molecular cloning and expression of 4-kb cDNA encoding the human urinary protein.
    Science. 1987 Mar 20;235(4795):1504-8 PMID: 3493529
  11. Expression of the M-CSF (CSF-1) gene by human monocytes.
    Blood. 1987 May;69(5):1409-13 PMID: 3105621
  12. Chemotactic activity of human recombinant granulocyte-macrophage colony-stimulating factor.
    Immunology. 1987 Mar;60(3):439-44 PMID: 3494669
  13. The human hematopoietic colony-stimulating factors.
    Science. 1987 Jun 5;236(4806):1229-37 PMID: 3296190
  14. Neutrophil chemotactic factor produced by lipopolysaccharide (LPS)-stimulated human blood mononuclear leukocytes: partial characterization and separation from interleukin 1 (IL 1).
    J Immunol. 1987 Aug 1;139(3):788-93 PMID: 3298433
  15. Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defense cytokines.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):9233-7 PMID: 3480540
  16. American Association of Pathologists president's address. New roles for the endothelium in inflammation and immunity.
    Am J Pathol. 1987 Dec;129(3):407-13 PMID: 3322019
  17. T-cells and macrophages in rapidly progressive glomerulonephritis: clinicopathologic correlations.
    Kidney Int. 1987 Dec;32(6):869-76 PMID: 3501499
  18. Biology of interleukin 1.
    FASEB J. 1988 Feb;2(2):108-15 PMID: 3277884
  19. The pathogenesis of atherosclerosis: atherogenesis and inflammation.
    Lab Invest. 1988 Mar;58(3):249-61 PMID: 3279259
  20. Expression of the macrophage colony-stimulating factor and c-fms genes in human acute myeloblastic leukemia cells.
    J Clin Invest. 1988 Apr;81(4):1030-5 PMID: 2832442
  21. A novel, NH2-terminal sequence-characterized human monokine possessing neutrophil chemotactic, skin-reactive, and granulocytosis-promoting activity.
    J Exp Med. 1988 Apr 1;167(4):1364-76 PMID: 3258625
  22. Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor.
    J Exp Med. 1988 Jun 1;167(6):1883-93 PMID: 3260265
  23. Regulation of the production and function of granulocytes and monocytes.
    Semin Hematol. 1988 Jul;25(3):173-88 PMID: 3043672
  24. Mesangial cells express PDGF mRNAs and proliferate in response to PDGF.
    Am J Physiol. 1988 Oct;255(4 Pt 2):F674-84 PMID: 2845810
  25. Monokine-induced gene expression of a human endothelial cell-derived neutrophil chemotactic factor.
    Biochem Biophys Res Commun. 1988 Nov 15;156(3):1340-5 PMID: 3263857
  26. IL-1 stimulates IL-6 production in endothelial cells.
    J Immunol. 1989 Jan 15;142(2):549-53 PMID: 2783442
  27. Granulocyte- and granulocyte-macrophage-colony stimulating factors induce human endothelial cells to migrate and proliferate.
    Nature. 1989 Feb 2;337(6206):471-3 PMID: 2464767
  28. Purification and amino acid analysis of two human glioma-derived monocyte chemoattractants.
    J Exp Med. 1989 Apr 1;169(4):1449-59 PMID: 2926329
  29. Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line.
    J Exp Med. 1989 Apr 1;169(4):1485-90 PMID: 2926331
  30. The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes.
    Science. 1989 Mar 17;243(4897):1464-6 PMID: 2648569
  31. Constitutive expression of the interleukin-6 gene in chronic lymphocytic leukemia.
    Blood. 1989 Apr;73(5):1279-84 PMID: 2784698
  32. The molecular control of cell division, differentiation commitment and maturation in haemopoietic cells.
    Nature. 1989 May 4;339(6219):27-30 PMID: 2469962
  33. The biology of interleukin-6.
    Blood. 1989 Jul;74(1):1-10 PMID: 2473791
  34. Multiple actions of interleukin 6 within a cytokine network.
    Immunol Today. 1988 May;9(5):137-9 PMID: 3076767
  35. Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils.
    J Clin Invest. 1989 Oct;84(4):1045-9 PMID: 2677047
  36. IgG1 plasmacytosis in interleukin 6 transgenic mice.
    Proc Natl Acad Sci U S A. 1989 Oct;86(19):7547-51 PMID: 2798426
  37. The neutrophil-activating peptide NAF/NAP-1 induces histamine and leukotriene release by interleukin 3-primed basophils.
    J Exp Med. 1989 Nov 1;170(5):1787-92 PMID: 2478657
  38. Macrophage inflammatory proteins 1 and 2: members of a novel superfamily of cytokines.
    FASEB J. 1989 Dec;3(14):2565-73 PMID: 2687068
  39. Identification by sequence analysis of chemotactic factors for monocytes produced by normal and transformed cells stimulated with virus, double-stranded RNA or cytokine.
    Eur J Immunol. 1989 Dec;19(12):2367-73 PMID: 2691259
  40. Cytokines as communication signals between leukocytes and endothelial cells.
    Immunol Today. 1989 Nov;10(11):370-5 PMID: 2514701
  41. A chemoattractant expressed in human sarcoma cells (tumor-derived chemotactic factor, TDCF) is identical to monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF).
    Int J Cancer. 1990 Apr 15;45(4):795-7 PMID: 2182547
  42. Monocyte chemotactic and activating factor gene expression induced in endothelial cells by IL-1 and tumor necrosis factor.
    J Immunol. 1990 Apr 15;144(8):3034-8 PMID: 2182712
  43. IL-1 transcriptionally activates the neutrophil chemotactic factor/IL-8 gene in endothelial cells.
    Immunology. 1990 Apr;69(4):548-53 PMID: 2185985
  44. Human monocyte chemoattractant protein-1 (MCP-1).
    Immunol Today. 1990 Mar;11(3):97-101 PMID: 2186747
  45. Induction of haptotactic migration of melanoma cells by neutrophil activating protein/interleukin-8.
    Biochem Biophys Res Commun. 1990 May 31;169(1):165-70 PMID: 2190552
  46. Tumor-associated macrophages.
    Curr Opin Immunol. 1989-1990;2(5):689-92 PMID: 2701968
  47. Mouse actin messenger RNAs. Construction and characterization of a recombinant plasmid molecule containing a complementary DNA transcript of mouse alpha-actin mRNA.
    J Biol Chem. 1981 Jan 25;256(2):1008-14 PMID: 6256358
  48. Cellular immunity in glomerulonephritis.
    Am J Pathol. 1982 Nov;109(2):227-43 PMID: 6753596
  49. Regulation of the macrophage content of neoplasms by chemoattractants.
    Science. 1983 Apr 8;220(4593):210-2 PMID: 6828888
  50. Macrophages and cellular immunity in experimental glomerulonephritis.
    Springer Semin Immunopathol. 1982;5(3):251-67 PMID: 6764037
  51. Cellular immune mechanisms in human glomerulonephritis: the role of mononuclear leucocytes.
    Springer Semin Immunopathol. 1982;5(3):269-96 PMID: 6764038
  52. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  53. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
    J Immunol Methods. 1983 Dec 16;65(1-2):55-63 PMID: 6606682
  54. Monoclonal antibody analysis of glomerular hypercellularity in human glomerulonephritis.
    Clin Nephrol. 1984 Oct;22(4):163-8 PMID: 6391767
  55. Molecular cloning of the complementary DNA for human tumor necrosis factor.
    Science. 1985 Apr 12;228(4696):149-54 PMID: 3856324
  56. Interleukin 1 and the glomerular mesangium. I. Purification and characterization of a mesangial cell-derived autogrowth factor.
    J Immunol. 1986 May 15;136(10):3700-5 PMID: 3486219
Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1991-04-00
Pages
991-1003
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1886116
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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