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PMID: 20154150 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

HSP70 and its cochaperone CPIP promote potyvirus infection in Nicotiana benthamiana by regulating viral coat protein functions.

The Plant cell ·Vol. 22 ·No. 2 ·2010-02-00 ·Pages 523-35

Hafrén A, Hofius D, Rönnholm G, Sonnewald U, Mäkinen K

Abstract

This study demonstrates that heat shock protein 70 (HSP70) together with its cochaperone CPIP regulates the function of a potyviral coat protein (CP), which in turn can interfere with viral gene expression. HSP70 was copurified as a component of a membrane-associated viral ribonucleoprotein complex from Potato virus A-infected plants. Downregulation of HSP70 caused a CP-mediated defect associated with replication. When PVA CP was expressed in trans, it interfered with viral gene expression and replication-associated translation (RAT). However, CP produced in cis interfered specifically with RAT. CPIP binds to potyviral CP, and overexpression of CPIP was sufficient to restore RAT inhibited by expression of CP in trans. Restoration of RAT was dependent on the ability of CPIP to interact with HSP70 since expression of a J-domain mutant, CPIP(Delta66), had only a minor effect on RAT. CPIP-mediated delivery of CP to HSP70 promoted CP degradation by increasing its ubiquitination when assayed in the absence of virus infection. In conclusion, CPIP and HSP70 are crucial components of a distinct translation activity that is associated with potyvirus replication.

MeSH Terms
Amino Acid Sequence Capsid Proteins/physiology Chromatography, Liquid Down-Regulation Gene Expression Regulation, Viral Genes, Viral HSP70 Heat-Shock Proteins/chemistry,physiology Molecular Chaperones/physiology Molecular Sequence Data Potyvirus/genetics,pathogenicity,physiology Protein Biosynthesis Tandem Mass Spectrometry Tobacco/virology Virus Replication
Chemicals
Capsid Proteins HSP70 Heat-Shock Proteins Molecular Chaperones
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hafrén Anders
Department of Applied Chemistry and Microbiology, University of Helsinki, 00014 Helsinki, Finland.
Hofius Daniel
Rönnholm Gunilla
Sonnewald Uwe
Mäkinen Kristiina
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.109.072413
Published
2010-02-00
Epub
2010-00-12
Pages
523-35
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2845415
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.109.072413 PMC Full Text View journal details
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