Home LiteratureArticle Details
PMID: 2017165 Published · ppublish English Comparative Study Journal Article

Transcriptional role of a conserved GATA-1 site in the human epsilon-globin gene promoter.

Molecular and cellular biology ·Vol. 11 ·No. 5 ·1991-05-00 ·Pages 2558-66

Gong QH, Stern J, Dean A

Abstract

The epsilon-globin gene is the first of the human beta-like globin genes to be expressed during development. We have analyzed protein-DNA interactions in the epsilon-globin promoter region by DNase I footprinting and electrophoretic mobility shift experiments using nuclear extracts from K562 human erythroid cells and from nonerythroid HeLa cells. A restricted set of ubiquitous proteins, including Sp1, bound to regions of the promoter including the CACCC and CCAAT sites. Three interactions, at positions -213, -165, and +3 relative to the transcription start site, were erythroid specific and corresponded to binding of GATA-1, a transcription factor highly restricted to the erythroid lineage. Interestingly, the GATA-1 site at -165 has been conserved in the promoters of 10 mammalian embryonic globin genes. Point mutations demonstrate that GATA-1 binding to this site is necessary for interaction with an erythroid-specific enhancer but that in the absence of an enhancer, GATA-1 does not increase transcription.

MeSH Terms
Animals Base Sequence Binding Sites Cell Line Chloramphenicol O-Acetyltransferase/genetics,metabolism DNA-Binding Proteins/metabolism Deoxyribonuclease I Erythroid-Specific DNA-Binding Factors GATA1 Transcription Factor Gene Expression Regulation Globins/genetics HeLa Cells/metabolism Humans Molecular Sequence Data Nucleotide Mapping Oligonucleotide Probes Plasmids Promoter Regions, Genetic Sequence Homology, Nucleic Acid Species Specificity Transcription Factors/metabolism Transcription, Genetic Transfection
Chemicals
DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors GATA1 Transcription Factor GATA1 protein, human Oligonucleotide Probes Transcription Factors Globins Chloramphenicol O-Acetyltransferase Deoxyribonuclease I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gong Q H
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Stern J
Dean A
References (50)
50 references, click to expand
  1. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  2. Cell type-specific protein-DNA interactions in the human zeta-globin upstream promoter region: displacement of Sp1 by the erythroid cell-specific factor NF-E1.
    Mol Cell Biol. 1990 Jan;10(1):282-94 PMID: 2403638
  3. Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit beta-globin gene in mouse 3T6 cells.
    Cell. 1983 Mar;32(3):695-706 PMID: 6299573
  4. Sequence and linkage of the goat epsilon I and epsilon II beta-globin genes.
    J Mol Biol. 1983 Sep 5;169(1):31-52 PMID: 6312053
  5. The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
    Cell. 1983 Nov;35(1):79-87 PMID: 6313230
  6. Molecular mechanisms of human hemoglobin switching: selective undermethylation and expression of globin genes in embryonic, fetal, and adult erythroblasts.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6907-11 PMID: 6316333
  7. Mapping of DNase I-hypersensitive sites in the upstream DNA of human embryonic epsilon-globin gene in K562 leukemia cells.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2718-22 PMID: 6232614
  8. The chromatin structure of the human epsilon globin gene: nuclease hypersensitive sites correlate with multiple initiation sites of transcription.
    Nucleic Acids Res. 1984 Dec 11;12(23):9191-204 PMID: 6096822
  9. A developmentally stable chromatin structure in the human beta-globin gene cluster.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1359-63 PMID: 3456593
  10. Fine structure genetic analysis of a beta-globin promoter.
    Science. 1986 May 2;232(4750):613-8 PMID: 3457470
  11. Regulated gene expression in transfected primary chicken erythrocytes.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4312-6 PMID: 3459175
  12. Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor.
    Cell. 1987 Jun 19;49(6):729-39 PMID: 3034432
  13. Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements.
    Cell. 1987 Jun 19;49(6):741-52 PMID: 3034433
  14. An enhancer element lies 3' to the human A gamma globin gene.
    EMBO J. 1987 Oct;6(10):2997-3004 PMID: 3691478
  15. In vitro binding of several cell-specific and ubiquitous nuclear proteins to the GT-I motif of the SV40 enhancer.
    Genes Dev. 1987 Oct;1(8):794-807 PMID: 2828172
  16. Cooperativity of the glucocorticoid receptor and the CACCC-box binding factor.
    Nature. 1988 Mar 3;332(6159):87-90 PMID: 2831456
  17. Human CCAAT-binding proteins have heterologous subunits.
    Cell. 1988 Apr 8;53(1):11-24 PMID: 3349524
  18. Bidirectional control of the chicken beta- and epsilon-globin genes by a shared enhancer.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2548-52 PMID: 3357880
  19. The human beta-globin gene contains multiple regulatory regions: identification of one promoter and two downstream enhancers.
    EMBO J. 1988 Feb;7(2):377-84 PMID: 2835225
  20. An erythroid specific nuclear factor binding to the proximal CACCC box of the beta-globin gene promoter.
    Nucleic Acids Res. 1988 May 25;16(10):4299-313 PMID: 2837728
  21. An erythrocyte-specific DNA-binding factor recognizes a regulatory sequence common to all chicken globin genes.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):5976-80 PMID: 3413070
  22. Developmental regulation of beta-globin gene switching.
    Cell. 1988 Oct 7;55(1):17-26 PMID: 3167976
  23. An erythrocyte-specific protein that binds to the poly(dG) region of the chicken beta-globin gene promoter.
    Genes Dev. 1988 Jul;2(7):863-73 PMID: 3209071
  24. Two tissue-specific factors bind the erythroid promoter of the human porphobilinogen deaminase gene.
    Nucleic Acids Res. 1989 Jan 11;17(1):37-54 PMID: 2911469
  25. Complete nucleotide sequence of the rabbit beta-like globin gene cluster. Analysis of intergenic sequences and comparison with the human beta-like globin gene cluster.
    J Mol Biol. 1989 Jan 5;205(1):15-40 PMID: 2486295
  26. Nucleotide sequence of the BALB/c mouse beta-globin complex.
    J Mol Biol. 1989 Jan 5;205(1):41-62 PMID: 2926808
  27. The human beta-globin promoter; nuclear protein factors and erythroid specific induction of transcription.
    EMBO J. 1988 Dec 20;7(13):4203-12 PMID: 3243278
  28. Nuclear proteins that bind the human gamma-globin gene promoter: alterations in binding produced by point mutations associated with hereditary persistence of fetal hemoglobin.
    Mol Cell Biol. 1988 Dec;8(12):5310-22 PMID: 2468996
  29. Nuclear protein factors and erythroid transcription of the human A gamma-globin gene.
    Nucleic Acids Res. 1989 May 25;17(10):3811-27 PMID: 2734104
  30. Identification of a transcriptional silencer in the 5'-flanking region of the human epsilon-globin gene.
    Proc Natl Acad Sci U S A. 1989 Jul;86(14):5306-9 PMID: 2748586
  31. Cis- and trans-acting elements involved in the regulation of the erythroid promoter of the human porphobilinogen deaminase gene.
    Proc Natl Acad Sci U S A. 1989 Sep;86(17):6548-52 PMID: 2771941
  32. The erythroid-specific transcription factor Eryf1: a new finger protein.
    Cell. 1989 Sep 8;58(5):877-85 PMID: 2776214
  33. The beta-globin stage selector element factor is erythroid-specific promoter/enhancer binding protein NF-E4.
    Genes Dev. 1989 Dec;3(12A):1845-59 PMID: 2620825
  34. A rapid method for site-specific mutagenesis and directional subcloning by using the polymerase chain reaction to generate recombinant circles.
    Biotechniques. 1990 Feb;8(2):178-83 PMID: 2180450
  35. Megakaryocytic and erythrocytic lineages share specific transcription factors.
    Nature. 1990 Mar 29;344(6265):447-9 PMID: 2320113
  36. A factor binding GATAAG confers tissue specificity on the promoter of the human zeta-globin gene.
    Nucleic Acids Res. 1990 Mar 25;18(6):1339-50 PMID: 2326182
  37. beta-globin dominant control region interacts differently with distal and proximal promoter elements.
    Genes Dev. 1990 Jun;4(6):1007-13 PMID: 2384211
  38. Developmentally regulated and erythroid-specific expression of the human embryonic beta-globin gene in transgenic mice.
    Nucleic Acids Res. 1990 Sep 25;18(18):5465-72 PMID: 2216720
  39. Globin gene regulation and switching: circa 1990.
    Cell. 1990 Nov 16;63(4):665-72 PMID: 2225071
  40. Interaction of epsilon-globin cis-acting control elements with erythroid-specific regulatory macromolecules.
    Proc Natl Acad Sci U S A. 1990 Oct;87(20):8115-9 PMID: 2236025
  41. Activity and tissue-specific expression of the transcription factor NF-E1 multigene family.
    Genes Dev. 1990 Oct;4(10):1650-62 PMID: 2249770
  42. Autonomous developmental control of human embryonic globin gene switching in transgenic mice.
    Science. 1990 Nov 23;250(4984):1147-9 PMID: 2251502
  43. Site-independent expression of the chicken beta A-globin gene in transgenic mice.
    Nature. 1990 Dec 20-27;348(6303):749-52 PMID: 2175398
  44. Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1.
    Nature. 1991 Jan 17;349(6306):257-60 PMID: 1987478
  45. Control of globin gene transcription.
    Annu Rev Cell Biol. 1990;6:95-124 PMID: 2275826
  46. Transcriptional activation and DNA binding by the erythroid factor GF-1/NF-E1/Eryf 1.
    Genes Dev. 1990 Nov;4(11):1886-98 PMID: 2276623
  47. trans-Activation of a globin promoter in nonerythroid cells.
    Mol Cell Biol. 1991 Feb;11(2):843-53 PMID: 1990287
  48. Mutational analysis of the chicken beta-globin enhancer reveals two positive-acting domains.
    Proc Natl Acad Sci U S A. 1988 Sep;85(17):6267-71 PMID: 3166139
  49. Binding of an erythroid-specific factor to enhancer regions of human globin genes.
    Prog Clin Biol Res. 1989;316A:179-91 PMID: 2594805
  50. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.5.2558-2566.1991
Published
1991-05-00
Pages
2558-66
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360025
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.11.5.2558-2566.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2