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PMID: 20211163 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Regulation of cell shape, wing hair initiation and the actin cytoskeleton by Trc/Fry and Wts/Mats complexes.

Developmental biology ·Vol. 341 ·No. 2 ·2010-05-15 ·Pages 360-74

Fang X, Adler PN

Abstract

The two NDR kinase family genes in Drosophila are tricornered (trc) and warts (wts). Previous studies on trc have focused on its role in the morphogenesis of extensions of epidermal cells and in dendrite branching and tiling. Studies on wts have focused on its roles as a tumor suppressor, in controlling photoreceptor type and in the maintenance of dendrites. Here we examine and compare the function of these genes in wing cells prior to their terminal differentiation. Mutations in these genes lead to changes in cell shape, cellular levels of F-actin, the timing of differentiation, and the expression of multiple wing hairs and DE-Cadherin. We showed that the effects of wts on all of these processes appear to be mediated by its regulation of the Yorkie transcription factor. We also provide evidence that trc regulates the expression of DE-cadherin and mwh. In addition, we showed that the effects on cell shape and the timing of differentiation appear to be not linked to changes in relative growth rate of cells compared to their neighbors.

MeSH Terms
Actins/metabolism Animals Cell Shape Cytoskeleton/metabolism Drosophila Proteins/metabolism Drosophila melanogaster/embryology Protein Kinases/metabolism Protein Serine-Threonine Kinases/metabolism Tumor Suppressor Proteins/metabolism Wings, Animal/metabolism
Chemicals
Actins Drosophila Proteins Mats protein, Drosophila Tumor Suppressor Proteins fry protein, Drosophila Protein Kinases trc protein, Drosophila wts protein, Drosophila Protein Serine-Threonine Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fang Xiaolan
Department of Biology, University of Virginia, Charlottesville, VA 22903, USA.
Adler Paul N
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Article Info
Journal
Developmental biology
Abbr.
Dev Biol
ISSN
1095-564X
DOI
10.1016/j.ydbio.2010.02.029
Published
2010-05-15
Epub
2010-00-06
Pages
360-74
Language
English
Region
United States
NLM ID
0372762
PMCID
PMC2862085
Subset
IM
Grants
NIGMS NIH HHS · R01 GM037163 · United States
NIGMS NIH HHS · R01 GM037163-24 · United States
NIGMS NIH HHS · R01 GM053498 · United States
NIGMS NIH HHS · R01 GM053498-08 · United States
External Links
PubMed source DOI: 10.1016/j.ydbio.2010.02.029 PMC Full Text View journal details
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