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PMID: 2023931 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Protein kinase A- and protein kinase C-regulated interaction of plectin with lamin B and vimentin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 9 ·1991-05-01 ·Pages 3812-6

Foisner R, Traub P, Wiche G

Abstract

Solid-phase binding assays with protein species purified from cultured rat glioma C6 cells and Ehrlich ascites revealed that plectin bound specifically to lamin B but not to lamins A and C. Lamin B interaction was significantly decreased upon in vitro phosphorylation of either lamin B or plectin with protein kinase A or C. In contrast, phosphorylation of plectin with kinase A increased its binding to vimentin, suggesting a different regulation of plectin interactions by this kinase. 32P-radiolabeling of rat glioma C6 cells revealed plectin as a major in vivo target of protein kinase A and protein kinase C. Plectin, present in lysates of dibutyryladenosine 3',5'-cyclic monophosphate-treated cells, showed a 2.5 times higher binding affinity to vimentin than plectin from phorbol ester-treated cells. Furthermore, the relative amounts of plectin in 1% Triton X-100/high salt-insoluble cell fractions decreased to one-fourth of control values upon treating cells with phorbol esters, whereas vimentin was unaffected. This finding suggested a protein kinase C-dependent weakening of plectin interaction with intermediate filaments in vivo. Taken together, these results point to a role of plectin in interlinking cytoskeletal and nuclear elements and suggest that specific protein kinases are involved in regulating these interactions.

MeSH Terms
Animals Blotting, Western In Vitro Techniques Intermediate Filament Proteins Intermediate Filaments/metabolism Lamin Type B Lamins Nuclear Envelope/metabolism Nuclear Proteins/metabolism Phosphorylation Plectin Protein Binding Protein Kinase C/physiology Protein Kinases/physiology Proteins/metabolism Rats Vimentin/metabolism
Chemicals
Intermediate Filament Proteins Lamin Type B Lamins Nuclear Proteins Plec protein, rat Plectin Proteins Vimentin Protein Kinases Protein Kinase C
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Foisner R
Institute of Biochemistry, University of Vienna, Austria.
Traub P
Wiche G
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42 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.9.3812
Published
1991-05-01
Pages
3812-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51543
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.9.3812 PMC Full Text View journal details
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