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PMID: 2023953 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The role of Tat in the human immunodeficiency virus life cycle indicates a primary effect on transcriptional elongation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 9 ·1991-05-01 ·Pages 4045-9

Feinberg MB, Baltimore D, Frankel AD

Abstract

The mechanism of Tat transactivation was studied by treating cell lines containing Tat-defective viruses with purified Tat protein. These cell lines constitutively produce very low levels of virus in the absence of Tat, as measured by p24 antigen levels. Virus production can be increased greater than 30,000-fold by adding exogenous Tat. Tat addition increases mRNA levels early in the viral life cycle, and Tat is required for Rev function to become evident. There is no evidence for a translational effect of Tat. Nuclear run-on experiments show that the increase in mRNA levels is due to an increased efficiency of elongation of nascent transcripts. These results suggest that Tat may be a gene-specific elongation factor.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Line Gene Expression Regulation, Viral Gene Products, tat/physiology Genetic Complementation Test HIV/genetics,growth & development HeLa Cells Humans In Vitro Techniques Molecular Sequence Data RNA, Viral/biosynthesis Time Factors Transcription, Genetic tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, tat RNA, Viral tat Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Feinberg M B
Whitehead Institute for Biomedical Research, Nine Cambridge Center, MA 02142.
Baltimore D
Frankel A D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.9.4045
Published
1991-05-01
Pages
4045-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51590
Subset
IM
Grants
NIAID NIH HHS · AI26463 · United States
NIAID NIH HHS · AI29135 · United States
NIGMS NIH HHS · GM39458 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.9.4045 PMC Full Text View journal details
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