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PMID: 2034682 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Protein geranylgeranyltransferase of Saccharomyces cerevisiae is specific for Cys-Xaa-Xaa-Leu motif proteins and requires the CDC43 gene product but not the DPR1 gene product.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 10 ·1991-05-15 ·Pages 4448-52

Finegold AA, Johnson DI, Farnsworth CC, Gelb MH, Judd SR, Glomset JA, Tamanoi F

Abstract

Protein prenylation occurs by modification of proteins with one of at least two isoprenoids, the farnesyl group and the geranylgeranyl group. Protein farnesyltransferases have been identified, but no such enzyme has been identified for geranylgeranylation. We report the identification of an activity in crude soluble yeast extracts that catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl pyrophosphate to proteins having the C-terminal sequence Cys-Ile-Ile-Leu or Cys-Val-Leu-Leu but not to a similar protein ending with Cys-Ile-Ile-Ser. This activity is dependent upon the CDC43/CAL1 gene, which is involved in budding and the control of cell polarity, but does not require the DPR1/RAM1 gene, which is known to be required for the farnesylation of Ras proteins. These results indicate that the protein geranylgeranyltransferase activity is distinct from the protein farnesyltransferase activity and that its specificity depends in part on the extreme C-terminal leucine in the protein to be prenylated.

Related Genes
CDC43 DPR1
MeSH Terms
Alkyl and Aryl Transferases Amino Acid Sequence Base Sequence Cell Cycle Proteins Fungal Proteins/genetics,metabolism Genes, Fungal Molecular Sequence Data Polyisoprenyl Phosphates/metabolism Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Substrate Specificity Transferases/metabolism
Chemicals
Cell Cycle Proteins Fungal Proteins Polyisoprenyl Phosphates Saccharomyces cerevisiae Proteins Transferases Alkyl and Aryl Transferases geranylgeranyltransferase type-I p21(ras) farnesyl-protein transferase CDC43 protein, S cerevisiae geranylgeranyl pyrophosphate
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Finegold A A
Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.
Johnson D I
Farnsworth C C
Gelb M H
Judd S R
Glomset J A
Tamanoi F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.10.4448
Published
1991-05-15
Pages
4448-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51677
Subset
IM
Grants
NCI NIH HHS · CA 087941 · United States
NCI NIH HHS · CA41996 · United States
NIGMS NIH HHS · GM 07183 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.10.4448 PMC Full Text View journal details
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