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PMID: 20375065 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Nano-topography sensing by osteoclasts.

Journal of cell science ·Vol. 123 ·No. Pt 9 ·2010-05-01 ·Pages 1503-10

Geblinger D, Addadi L, Geiger B

Abstract

Bone resorption by osteoclasts depends on the assembly of a specialized, actin-rich adhesive 'sealing zone' that delimits the area designed for degradation. In this study, we show that the level of roughness of the underlying adhesive surface has a profound effect on the formation and stability of the sealing zone and the associated F-actin. As our primary model substrate, we use 'smooth' and 'rough' calcite crystals with average topography values of 12 nm and 530 nm, respectively. We show that the smooth surfaces induce the formation of small and unstable actin rings with a typical lifespan of approximately 8 minutes, whereas the sealing zones formed on the rough calcite surfaces are considerably larger, and remain stable for more than 6 hours. It was further observed that steps or sub-micrometer cracks on the smooth surface stimulate local ring formation, raising the possibility that similar imperfections on bone surfaces may stimulate local osteoclast resorptive activity. The mechanisms whereby the physical properties of the substrate influence osteoclast behavior and their involvement in osteoclast function are discussed.

MeSH Terms
Absorption/drug effects Animals Biosensing Techniques/methods Calcium Carbonate/pharmacology Cell Line Humans Mice Mice, Inbred C57BL Nanostructures/chemistry Osteoclasts/cytology,drug effects,metabolism Surface Properties/drug effects Time Factors Vitronectin/metabolism,pharmacology
Chemicals
Vitronectin Calcium Carbonate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Geblinger Dafna
Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel.
Addadi Lia
Geiger Benjamin
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Article Info
Journal
Journal of cell science
Abbr.
J Cell Sci
ISSN
1477-9137
DOI
10.1242/jcs.060954
Published
2010-05-01
Epub
2010-00-07
Pages
1503-10
Language
English
Region
England
NLM ID
0052457
PMCID
PMC2858020
Subset
IM
Grants
NIGMS NIH HHS · U54 GM064346 · United States
NIGMS NIH HHS · U54GM64346 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1242/jcs.060954 PMC Full Text View journal details
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