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PMID: 20395279 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In silico analysis reveals 75 members of mitogen-activated protein kinase kinase kinase gene family in rice.

DNA research : an international journal for rapid publication of reports on genes and genomes ·Vol. 17 ·No. 3 ·2010-06-00 ·Pages 139-53

Rao KP, Richa T, Kumar K, Raghuram B, Sinha AK

Abstract

Mitogen-Activated Protein Kinase Kinase Kinases (MAPKKKs) are important components of MAPK cascades, which are universal signal transduction modules and play important role in plant growth and development. In the sequenced Arabidopsis genome 80 MAPKKKs were identified and currently being analysed for its role in different stress. In rice, economically important monocot cereal crop only five MAPKKKs were identified so far. In this study using computational analysis of sequenced rice genome we have identified 75 MAPKKKs. EST hits and full-length cDNA sequences (from KOME or Genbank database) of 75 MAPKKKs supported their existence. Phylogenetic analyses of MAPKKKs from rice and Arabidopsis have classified them into three subgroups, which include Raf, ZIK and MEKK. Conserved motifs in the deduced amino acid sequences of rice MAPKKKs strongly supported their identity as members of Raf, ZIK and MEKK subfamilies. Further expression analysis of the MAPKKKs in MPSS database revealed that their transcripts were differentially regulated in various stress and tissue-specific libraries.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics Computational Biology Gene Expression Regulation, Plant Genome, Plant MAP Kinase Kinase Kinases/classification,genetics Molecular Sequence Data Oryza/genetics Phylogeny Plant Proteins/genetics Sequence Homology, Amino Acid Species Specificity
Chemicals
Plant Proteins MAP Kinase Kinase Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rao Kudupudi Prabhakara
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
Richa Tambi
Kumar Kundan
Raghuram Badmi
Sinha Alok Krishna
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Article Info
Journal
DNA research : an international journal for rapid publication of reports on genes and genomes
Abbr.
DNA Res
ISSN
1756-1663
DOI
10.1093/dnares/dsq011
Published
2010-06-00
Epub
2010-00-15
Pages
139-53
Language
English
Region
England
NLM ID
9423827
PMCID
PMC2885274
Subset
IM
External Links
PubMed source DOI: 10.1093/dnares/dsq011 PMC Full Text View journal details
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