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PMID: 20400559 Published · ppublish English Evaluation Study Journal Article

Identification and typing of Lactococcus lactis by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Applied and environmental microbiology ·Vol. 76 ·No. 12 ·2010-06-00 ·Pages 4055-62

Tanigawa K, Kawabata H, Watanabe K

Abstract

Currently, the genus Lactococcus is classified into six species: Lactococcus chungangensis, L. garvieae, L. lactis, L. piscium, L. plantarum, and L. raffinolactis. Among these six species, L. lactis is especially important because of its use in the manufacture of probiotic dairy products. L. lactis consists of three subspecies: L. lactis subsp. cremoris, L. lactis subsp. hordniae, and L. lactis subsp. lactis. However, these subspecies have not yet been reliably discriminated. To date, mainly phenotypic identification has been used, with a few genotypic identifications. We discriminated species or subspecies in the genus Lactococcus not only by proteomics identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) but also by phenotypic and genotypic identification. The proteomics identification using differences in the mass spectra of ribosomal proteins was nearly identical to that by genotypic identification (i.e., by analyses of 16S rRNA and recA gene sequences and amplified fragment length polymorphism). The three ribosomal subunits 30S/L31, 50S/L31, and 50S/L35 were the best markers for discriminating L. lactis subsp. cremoris from L. lactis subsp. lactis. Proteomics identification using MALDI-TOF MS was therefore a powerful method for discriminating and identifying these bacteria. In addition, this method was faster and more reliable than others that we examined.

MeSH Terms
Bacterial Proteins/analysis,genetics Bacterial Typing Techniques/methods Genotype Lactococcus lactis/chemistry,classification,genetics,isolation & purification RNA, Ribosomal, 16S/genetics Rec A Recombinases/genetics Ribosomal Proteins/analysis,genetics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Chemicals
Bacterial Proteins RNA, Ribosomal, 16S Ribosomal Proteins Rec A Recombinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tanigawa Kana
Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186-8650, Japan. [email protected]
Kawabata Hiroaki
Watanabe Koichi
References (35)
35 references, click to expand
  1. Identification of lactobacilli by pheS and rpoA gene sequence analyses.
    Int J Syst Evol Microbiol. 2007 Dec;57(Pt 12):2777-2789 PMID: 18048724
  2. PCR amplification of the gene acmA differentiates Lactococcus lactis subsp. lactis and L. lactis subsp. cremoris.
    Appl Environ Microbiol. 1999 Nov;65(11):5151-3 PMID: 10543836
  3. Development and application of oligonucleotide probes for identification of Lactococcus lactis subsp. cremoris.
    Appl Environ Microbiol. 1991 May;57(5):1313-8 PMID: 1713027
  4. Multilocus sequence typing of Lactobacillus casei reveals a clonal population structure with low levels of homologous recombination.
    Appl Environ Microbiol. 2007 Oct;73(20):6601-11 PMID: 17704267
  5. Two methods for the genetic differentiation of Lactococcus lactis ssp. lactis and cremoris based on differences in the 16S rRNA gene sequence.
    FEMS Microbiol Lett. 1998 Sep 1;166(1):15-20 PMID: 9741080
  6. Rapid identification and typing of listeria species by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    Appl Environ Microbiol. 2008 Sep;74(17):5402-7 PMID: 18606788
  7. Microorganism identification by matrix-assisted laser/desorption ionization mass spectrometry and model-derived ribosomal protein biomarkers.
    Anal Chem. 2003 Aug 1;75(15):3817-22 PMID: 14572048
  8. Rapid PCR-based method which can determine both phenotype and genotype of Lactococcus lactis subspecies.
    Appl Environ Microbiol. 2002 May;68(5):2209-13 PMID: 11976090
  9. Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria.
    J Clin Microbiol. 2008 Jun;46(6):1946-54 PMID: 18400920
  10. Isolation of Lactococcus lactis subsp. cremoris from nature by colony hybridization with rRNA probes.
    Appl Environ Microbiol. 1993 Nov;59(11):3941-5 PMID: 7506898
  11. Divergence of Genomic Sequences between Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris.
    Appl Environ Microbiol. 1992 Dec;58(12):4045-7 PMID: 16348830
  12. The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403.
    Genome Res. 2001 May;11(5):731-53 PMID: 11337471
  13. Genotypic vs. phenotypic biodiversity in Lactococcus lactis.
    Microbiology (Reading). 2002 Nov;148(Pt 11):3332-3333 PMID: 12427924
  14. Rapid identification of viridans streptococci by mass spectrometric discrimination.
    J Clin Microbiol. 2007 Aug;45(8):2392-7 PMID: 17553974
  15. Phenotypic and molecular characterization of Lactococcus lactis from milk and plants.
    J Appl Microbiol. 2006 Aug;101(2):396-405 PMID: 16882147
  16. The ldh phylogeny for environmental isolates of Lactococcus lactis is consistent with rRNA genotypes but not with phenotypes.
    Appl Environ Microbiol. 1997 Feb;63(2):694-702 PMID: 9023947
  17. Amplified fragment length polymorphism of Streptococcus suis strains correlates with their profile of virulence-associated genes and clinical background.
    J Med Microbiol. 2007 Jan;56(Pt 1):102-109 PMID: 17172524
  18. Comparative sequence analysis of a recA gene fragment brings new evidence for a change in the taxonomy of the Lactobacillus casei group.
    Int J Syst Evol Microbiol. 2001 Nov;51(Pt 6):2113-2117 PMID: 11760954
  19. Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing.
    J Bacteriol. 1983 Apr;154(1):1-9 PMID: 6403500
  20. Discrimination of intact mycobacteria at the strain level: a combined MALDI-TOF MS and biostatistical analysis.
    Proteomics. 2006 Dec;6(24):6416-25 PMID: 17109381
  21. Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride.
    Nucleic Acids Res. 1993 Nov 11;21(22):5279-80 PMID: 8255788
  22. The neighbor-joining method: a new method for reconstructing phylogenetic trees.
    Mol Biol Evol. 1987 Jul;4(4):406-25 PMID: 3447015
  23. Treatment of murine colitis by Lactococcus lactis secreting interleukin-10.
    Science. 2000 Aug 25;289(5483):1352-5 PMID: 10958782
  24. Speciation of Campylobacter coli, C. jejuni, C. helveticus, C. lari, C. sputorum, and C. upsaliensis by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
    Appl Environ Microbiol. 2005 Oct;71(10):6292-307 PMID: 16204551
  25. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801-5 PMID: 368799
  26. A simple intact protein analysis by MALDI-MS for characterization of ribosomal proteins of two genome-sequenced lactic acid bacteria and verification of their amino acid sequences.
    J Proteome Res. 2007 Oct;6(10):3899-907 PMID: 17854216
  27. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.
    Mol Biol Evol. 2007 Aug;24(8):1596-9 PMID: 17488738
  28. Rapid classification and identification of salmonellae at the species and subspecies levels by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry .
    Appl Environ Microbiol. 2008 Dec;74(24):7767-78 PMID: 18952875
  29. Deoxyribonucleic Acid homology among lactic streptococci.
    Appl Environ Microbiol. 1981 Jan;41(1):77-83 PMID: 16345703
  30. Novel characteristic for distinguishing Lactococcus lactis subsp. lactis from subsp. cremoris.
    Int J Syst Bacteriol. 1999 Jan;49 Pt 1:163-6 PMID: 10028257
  31. Integrated polymerase chain reaction-based procedures for the detection and identification of species and subspecies of the Gram-positive bacterial genus Lactococcus.
    J Appl Microbiol. 2002;93(2):353-61 PMID: 12147085
  32. Complete genome sequence of the prototype lactic acid bacterium Lactococcus lactis subsp. cremoris MG1363.
    J Bacteriol. 2007 Apr;189(8):3256-70 PMID: 17307855
  33. Cloning, sequencing and comparison of three lactococcal L-lactate dehydrogenase genes.
    Microbiology (Reading). 1994 Jun;140 ( Pt 6):1301-5 PMID: 8081494
  34. Diversity analysis of dairy and nondairy Lactococcus lactis isolates, using a novel multilocus sequence analysis scheme and (GTG)5-PCR fingerprinting.
    Appl Environ Microbiol. 2007 Nov;73(22):7128-37 PMID: 17890345
  35. Comparative genomics of the lactic acid bacteria.
    Proc Natl Acad Sci U S A. 2006 Oct 17;103(42):15611-6 PMID: 17030793
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
DOI
10.1128/AEM.02698-09
Email
[email protected]
Published
2010-06-00
Epub
2010-00-16
Pages
4055-62
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC2893499
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.02698-09 PMC Full Text View journal details
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