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PMID: 2046658 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

New classes of Streptomyces coelicolor A3(2) mutants blocked in undecylprodigiosin (Red) biosynthesis.

Molecular & general genetics : MGG ·Vol. 227 ·No. 1 ·1991-05-00 ·Pages 28-32

Coco EA, Narva KE, Feitelson JS

Abstract

Fifteen mutants of Streptomyces coelicolor A3(2) blocked in both the bipyrrole branch (redA) and a second site specific to the undecylprodigiosin pathway were characterized. Some of the mutants were ordered biosynthetically based on cosynthesis experiments. Complementation of each of the mutants with wild-type DNA cloned in low- and high-copy number plasmid vectors allowed the mutants to be separated into 12 new classes which are physically clustered within approximately 37 kb on the S. coelicolor genome. Early-step biosynthetic genes are centrally located and are flanked by later-step and regulatory genes.

MeSH Terms

DNA, Bacterial/genetics,isolation & purification Genetic Complementation Test Mutation Plasmids Prodigiosin/analogs & derivatives,biosynthesis Streptomyces/genetics,isolation & purification,metabolism

Chemicals

DNA, Bacterial undecylprodigiosin Prodigiosin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Coco E A

Medical Research Division, American Cyanamid Company, Pearl River, NY 10965.

Narva K E

Feitelson J S

References (12)

12 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00260702

Published

1991-05-00

Pages

28-32

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00260702

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New classes of Streptomyces coelicolor A3(2) mutants blocked in undecylprodigiosin (Red) biosynthesis.

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