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PMID: 2050109 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Manganese superoxide dismutase can reduce cellular damage mediated by oxygen radicals in transgenic plants.

The EMBO journal ·Vol. 10 ·No. 7 ·1991-07-00 ·Pages 1723-32

Bowler C, Slooten L, Vandenbranden S, De Rycke R, Botterman J, Sybesma C, Van Montagu M, Inzé D

Abstract

In plants, environmental adversity often leads to the formation of highly reactive oxygen radicals. Since resistance to such conditions may be correlated with the activity of enzymes involved in oxygen detoxification, we have generated transgenic tobacco plants which express elevated levels of manganese superoxide dismutase (MnSOD) within their chloroplasts or mitochondria. Leaf discs of these plants have been analyzed in conditions in which oxidative stress was generated preferentially within one or the other organelle. It was found that high level overproduction of MnSOD in the corresponding subcellular location could significantly reduce the amount of cellular damage which would normally occur. In contrast, small increases in MnSOD activity were deleterious under some conditions. A generally applicable model correlating the consequences of SOD with the magnitude of its expression is presented.

MeSH Terms
Amino Acid Sequence Base Sequence Chimera Chloroplasts/drug effects,enzymology DNA/chemistry Free Radicals Mitochondria/drug effects,enzymology Molecular Sequence Data Oxygen/toxicity Paraquat/pharmacology Plants, Toxic Superoxide Dismutase/biosynthesis,genetics Tobacco/enzymology,genetics Transformation, Genetic
Chemicals
Free Radicals DNA Superoxide Dismutase Paraquat Oxygen
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Bowler C
Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.
Slooten L
Vandenbranden S
De Rycke R
Botterman J
Sybesma C
Van Montagu M
Inzé D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-07-00
Pages
1723-32
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452843
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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