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PMID: 20505758 Published · epublish English Journal Article Research Support, N.I.H., Intramural

microRNA expression patterns reveal differential expression of target genes with age.

PloS one ·Vol. 5 ·No. 5 ·2010-05-20 ·Pages e10724

Noren Hooten N, Abdelmohsen K, Gorospe M, Ejiogu N, Zonderman AB, Evans MK

Abstract

Recent evidence supports a role for microRNAs (miRNAs) in regulating the life span of model organisms. However, little is known about how these small RNAs contribute to human aging. Here, we profiled the expression of over 800 miRNAs in peripheral blood mononuclear cells from young and old individuals by real-time RT-PCR analysis. This genome-wide assessment of miRNA expression revealed that the majority of miRNAs studied decreased in abundance with age. We identified nine miRNAs (miR-103, miR-107, miR-128, miR-130a, miR-155, miR-24, miR-221, miR-496, miR-1538) that were significantly lower in older individuals. Among them, five have been implicated in cancer pathogenesis. Predicted targets of several of these miRNAs, including PI3 kinase (PI3K), c-Kit and H2AX, were found to be elevated with advancing age, supporting a possible role for them in the aging process. Furthermore, we found that decreasing the levels of miR-221 was sufficient to cause a corresponding increase in the expression of the predicted target, PI3K. Taken together, these findings demonstrate that changes in miRNA expression occur with human aging and suggest that miRNAs and their predicted targets have the potential to be diagnostic indicators of age or age-related diseases.

MeSH Terms
Adult Aging/genetics Animals Base Sequence Caenorhabditis elegans/genetics,growth & development Down-Regulation/genetics Female Gene Expression Profiling Gene Expression Regulation, Developmental HeLa Cells Humans Male MicroRNAs/genetics,metabolism Middle Aged Molecular Sequence Data Phosphatidylinositol 3-Kinases/genetics,metabolism Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction Signal Transduction/genetics Up-Regulation/genetics
Chemicals
MicroRNAs Phosphatidylinositol 3-Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Noren Hooten Nicole
Laboratory of Cellular and Molecular Biology, National Institute on Aging, National Institutes of Health, Baltimore, Maryland, USA.
Abdelmohsen Kotb
Gorospe Myriam
Ejiogu Ngozi
Zonderman Alan B
Evans Michele K
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0010724
Published
2010-05-20
Epub
2010-00-20
Pages
e10724
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC2873959
Subset
IM
Grants
Intramural NIH HHS · United States
External Links
PubMed source DOI: 10.1371/journal.pone.0010724 PMC Full Text View journal details
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