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PMID: 20603003 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Arabidopsis HEMERA/pTAC12 initiates photomorphogenesis by phytochromes.

Cell ·Vol. 141 ·No. 7 ·2010-06-25 ·Pages 1230-40

Chen M, Galvão RM, Li M, Burger B, Bugea J, Bolado J, Chory J

Abstract

Light plays a profound role in plant development, yet how photoreceptor excitation directs phenotypic plasticity remains elusive. One of the earliest effects of light is the regulated translocation of the red/far-red photoreceptors, phytochromes, from the cytoplasm to subnuclear foci called phytochrome nuclear bodies. The function of these nuclear bodies is unknown. We report the identification of hemera, a seedling lethal mutant of Arabidopsis with altered phytochrome nuclear body patterns. hemera mutants are impaired in all phytochrome responses examined, including proteolysis of phytochrome A and phytochrome-interacting transcription factors. HEMERA was identified previously as pTAC12, a component of a plastid complex associated with transcription. Here, we show that HEMERA has a function in the nucleus, where it acts specifically in phytochrome signaling, is predicted to be structurally similar to the multiubiquitin-binding protein, RAD23, and can partially rescue yeast rad23mutants. Together, these results implicate phytochrome nuclear bodies as sites of proteolysis.

MeSH Terms
Arabidopsis/cytology,growth & development,metabolism Arabidopsis Proteins/metabolism Cell Nucleus/metabolism Chloroplasts/metabolism DNA-Binding Proteins/metabolism Green Fluorescent Proteins/analysis Intracellular Signaling Peptides and Proteins Light Microscopy, Confocal Nuclear Proteins/metabolism Phytochrome A/metabolism Phytochrome B/metabolism Saccharomyces cerevisiae Proteins/metabolism Seeds/metabolism Signal Transduction Transcription Factors/metabolism
Chemicals
Arabidopsis Proteins DET1 protein, Arabidopsis DNA-Binding Proteins HMR protein, Arabidopsis Intracellular Signaling Peptides and Proteins Nuclear Proteins PHYB protein, Arabidopsis Phytochrome A RAD23 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Phytochrome B Green Fluorescent Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Chen Meng
Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA. [email protected] <[email protected]>
Galvão Rafaelo M
Li Meina
Burger Brian
Bugea Jane
Bolado Jack
Chory Joanne
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2010.05.007
Email
[email protected]
Published
2010-06-25
Pages
1230-40
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2935685
Subset
IM
Grants
NIGMS NIH HHS · R01 GM052413 · United States
NIGMS NIH HHS · R01 GM052413-16 · United States
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · R01GM52413 · United States
External Links
PubMed source DOI: 10.1016/j.cell.2010.05.007 PMC Full Text View journal details
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