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PMID: 2062838 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Delivery of macromolecules into living cells: a method that exploits folate receptor endocytosis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 13 ·1991-07-01 ·Pages 5572-6

Leamon CP, Low PS

Abstract

Difficulties with the nondestructive delivery of macromolecules into living cells have limited the potential applications of antibodies, genes, enzymes, peptides, and antisense oligonucleotides in biology and medicine. We have found, however, that the natural endocytosis pathway for the vitamin folate can be exploited to nondestructively introduce macromolecules into cultured cells if the macromolecule is first covalently linked to folate. Thus, treatment of KB cells with folate-conjugated ribonuclease, horseradish peroxidase, serum albumin, IgG, or ferritin allowed delivery of greater than 10(6) copies of the macromolecules within a 2-hr period. Cytochemical staining using 4-chloro-1-naphthol further demonstrated that the horseradish peroxidase retained activity for at least 6 hr after internalization. Since folate is an essential vitamin required in substantial quantities by virtually all cells, these observations may open the possibility of scientific and medical applications for many of the above macromolecules.

MeSH Terms
Carrier Proteins/physiology Cell Line Endocytosis Folate Receptors, GPI-Anchored Folic Acid/chemistry,metabolism Horseradish Peroxidase/chemistry Humans Immunoglobulin G/chemistry Proteins/chemistry,metabolism Receptors, Cell Surface Ribonuclease, Pancreatic/chemistry Serum Albumin, Bovine/chemistry Time Factors
Chemicals
Carrier Proteins Folate Receptors, GPI-Anchored Immunoglobulin G Proteins Receptors, Cell Surface Serum Albumin, Bovine Folic Acid Horseradish Peroxidase Ribonuclease, Pancreatic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Leamon C P
Department of Chemistry, Purdue University, West Lafayette, IN 47907.
Low P S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.13.5572
Published
1991-07-01
Pages
5572-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51919
Subset
IM
Grants
NIGMS NIH HHS · GM08296 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.13.5572 PMC Full Text View journal details
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