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PMID: 2062864 Published · ppublish English Journal Article

Spermidine or spermine is essential for the aerobic growth of Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 13 ·1991-07-01 ·Pages 5872-6

Balasundaram D, Tabor CW, Tabor H

Abstract

A null mutation in the SPE2 gene of Saccharomyces cerevisiae, encoding S-adenosylmethionine decarboxylase, results in cells with no detectable S-adenosylmethionine decarboxylase, spermidine, and spermine. This mutant has an absolute requirement for spermidine or spermine for growth; this requirement is not satisfied by putrescine. Polyamine-depleted cells show a number of microscopic abnormalities that are similar to those reported for several cell division cycle (cdc) and actin mutants. These include a striking increase in cell size, a marked decrease in budding, accumulation of vesicle-like bodies, absence of specific localization of chitin-like material, and abnormal distribution of actin-like material. The absolute requirement for polyamines for growth and the microscopic abnormalities are not seen if the cultures are grown under anaerobic conditions.

Related Genes
SPE2
MeSH Terms
Adenosylmethionine Decarboxylase/metabolism Aerobiosis Anaerobiosis DNA Mutational Analysis Genes, Fungal Saccharomyces cerevisiae/cytology,genetics,growth & development Spermidine/physiology Spermine/physiology
Chemicals
Spermine Adenosylmethionine Decarboxylase Spermidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Balasundaram D
Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
Tabor C W
Tabor H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.13.5872
Published
1991-07-01
Pages
5872-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51980
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.13.5872 PMC Full Text View journal details
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