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PMID: 2068100 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular cloning of matrin F/G: A DNA binding protein of the nuclear matrix that contains putative zinc finger motifs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 14 ·1991-07-15 ·Pages 6186-90

Hakes DJ, Berezney R

Abstract

We have isolated a 2.7-kilobase rat liver cDNA clone that contains the entire 544-amino acid coding sequence for matrin F/G. This protein has previously been localized to the internal, fibrogranular areas of the nuclear matrix and shown to bind to DNA on nitrocellulose blots. The predicted amino acid sequence from the coding region of this cDNA shows that this protein contains approximately 50% hydrophobic amino acids with secondary structure predictions suggesting a large percentage of beta-sheet regions. No significant homologies were found with any other known proteins, including the nuclear lamins. The predicted amino acid sequence was also searched for DNA binding motifs. Two putative zinc finger motifs were found. In addition, a 7-mer palindromic sequence (Ser-Ser-Thr-Asn-Thr-Ser-Ser) was discovered within one of these zinc finger DNA binding regions. A possible regulatory role for this element is discussed.

MeSH Terms
Amino Acid Sequence Animals Antiporters Base Sequence Binding Sites DNA/genetics,isolation & purification DNA-Binding Proteins/genetics Gene Library Liver/physiology Molecular Sequence Data Nuclear Matrix/physiology Organic Anion Transporters Protein Conformation Rats Restriction Mapping Zinc Fingers/genetics
Chemicals
Antiporters DNA-Binding Proteins Organic Anion Transporters Slco2a1 protein, rat DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hakes D J
Department of Biological Sciences, State University of New York, Buffalo 14260.
Berezney R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.14.6186
Published
1991-07-15
Pages
6186-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52047
Subset
IM
Grants
NIGMS NIH HHS · GM-23922 · United States
Databases
GENBANK
M60981, M60982, M60983, M60984, M60985, M60986, M60987, M60988, M64862, M76989
External Links
PubMed source DOI: 10.1073/pnas.88.14.6186 PMC Full Text View journal details
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