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PMID: 3016715 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of two related Epstein-Barr virus-encoded membrane proteins that are differentially expressed in Burkitt lymphoma and in vitro-transformed cell lines.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 15 ·1986-08-00 ·Pages 5703-7

Modrow S, Wolf H

Abstract

Two related but differentially expressed potential membrane proteins of Epstein-Barr virus are encoded by the same reading frame in the EcoRI D het region of the viral genome. Potential antigenic sites in the amino acid sequence of these proteins were selected by computer-aided prediction of the secondary structure and two oligopeptides corresponding to regions located in different parts of the proteins were synthesized chemically. Rabbit antisera to these peptides were used for immunoprecipitation of the native viral proteins from Epstein-Barr virus-positive cell lines from various sources. Both predicted membrane proteins could be precipitated from cell lines that had been transformed in vitro with EBV or from cell lines derived from infectious mononucleosis patients. In cell lines established from Burkitt lymphoma, only the smaller polypeptide, which lacks 138 amino acids from the amino terminus, could be identified. Using the synthetic peptides as antigens in ELISA, we detected elevated antibody titers in sera from patients with infectious mononucleosis and nasopharyngeal carcinoma.

MeSH Terms
Amino Acid Sequence Antigens, Viral/analysis Burkitt Lymphoma/immunology Cell Line Cell Transformation, Viral Chemical Precipitation Enzyme-Linked Immunosorbent Assay Herpesvirus 4, Human/immunology,metabolism Humans Membrane Proteins/immunology,metabolism Molecular Weight Oligopeptides/chemical synthesis,immunology Viral Proteins/immunology,metabolism
Chemicals
Antigens, Viral Membrane Proteins Oligopeptides Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Modrow S
Wolf H
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.15.5703
Published
1986-08-00
Pages
5703-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386357
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.15.5703 PMC Full Text View journal details
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