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PMID: 6095274 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A membrane protein encoded by Epstein-Barr virus in latent growth-transforming infection.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 22 ·1984-11-00 ·Pages 7207-11

Hennessy K, Fennewald S, Hummel M, Cole T, Kieff E

Abstract

The nucleotide sequence of an Epstein-Barr virus gene expressed in latently infected growth-transformed cells is known to include a long open reading frame containing a 33-base-pair repeat element. A bacterial fusion protein constructed from a portion of the reading frame and Escherichia coli beta-galactosidase was used to produce sera in rabbits against the previously unidentified gene product. The viral protein detected with these sera in latently infected cells varies in size with the number of copies of the DNA repeat element. Translation of the RNA in vitro yields a protein of similar size. As expected from its primary sequence, the protein is a membrane protein. Immunofluorescence studies with the rabbit antisera suggest that the protein is in the plasma membrane. Thus, this protein could be the lymphocyte-determined membrane antigen (LYDMA) responsible for the generation of T-cell immunity to latently infected cells.

MeSH Terms
Cell Membrane/immunology Cell Transformation, Viral Escherichia coli Herpesvirus 4, Human/genetics,immunology Membrane Proteins/genetics,immunology Molecular Weight Protein Conformation Solubility Viral Proteins/genetics,immunology
Chemicals
Membrane Proteins Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hennessy K
Fennewald S
Hummel M
Cole T
Kieff E
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.22.7207
Published
1984-11-00
Pages
7207-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392107
Subset
IM
Grants
NCI NIH HHS · CA 17281 · United States
NCI NIH HHS · CA 19264 · United States
NIGMS NIH HHS · GM 07183 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.22.7207 PMC Full Text View journal details
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