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PMID: 2072462 Published · ppublish English Journal Article

The indirect association of human T-cell leukemia virus tax protein with DNA results in transcriptional activation.

Journal of virology ·Vol. 65 ·No. 8 ·1991-08-00 ·Pages 4525-8

Fujisawa J, Toita M, Yoshimura T, Yoshida M

Abstract

trans-activator p40tax of human T-cell leukemia virus type I activates specific enhancers and stimulates transcription of the viral and some cellular genes but does not bind directly to the enhancer sequences. We demonstrated here that a fusion protein of p40tax and GAL4 DNA binding domain activated transcription dependent on the GAL4-binding site in the reporter plasmid. Mutants of p40tax were also tested in the fusion protein, and their activities were found to be in parallel with those of their free forms on the original target long terminal repeat. This activation with GAL4 fusion protein was interfered with by the free form of p40tax. These results suggest that p40tax associates with DNA through interaction with DNA binding protein(s) and also interacts with another transcription factor(s) to elicit the activation.

Related Genes
tax
MeSH Terms
Blotting, Western Cell Line Chloramphenicol O-Acetyltransferase/genetics DNA, Viral/metabolism DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Gene Expression Regulation, Viral Gene Products, tax/metabolism Human T-lymphotropic virus 1/genetics Humans Mutagenesis Plasmids Recombinant Fusion Proteins/metabolism Transcription, Genetic Transcriptional Activation Transfection
Chemicals
DNA, Viral DNA-Binding Proteins Gene Products, tax Recombinant Fusion Proteins Chloramphenicol O-Acetyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fujisawa J
Department of Cellular and Molecular Biology, University of Tokyo, Japan.
Toita M
Yoshimura T
Yoshida M
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34 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.8.4525-4528.1991
Published
1991-08-00
Pages
4525-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC248898
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.65.8.4525-4528.1991 PMC Full Text View journal details
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