Neutral metallo-proteinases of rabbit bone. Separation in latent forms of distinct enzymes that when activated degrade collagen, gelatin and proteoglycans.

The Biochemical journal ·Vol. 171 ·No. 2 ·1978-05-01 ·Pages 493-6

Sellers A, Reynolds JJ, Meikle MC

Abstract

Rabbit bones in culture produce specific collagenase and neutral metallo-proteinase activity in latent forms that can be activated by either 4-aminophenylmercuric acetate or trypsin. Latent neutral metallo-proteinase activity was resolved by gel filtration into two enzymes, distinct from collagenase, that degrade gelatin and cartilage proteoglycans.

MeSH Terms

Animals Bone and Bones/enzymology Collagen/metabolism Endopeptidases/metabolism Enzyme Activation Gelatin/metabolism Metalloproteins/metabolism Microbial Collagenase/metabolism Organ Culture Techniques Proteoglycans/metabolism Rabbits

Chemicals

Metalloproteins Proteoglycans Gelatin Collagen Endopeptidases Microbial Collagenase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sellers A

Reynolds J J

Meikle M C

References (11)

11 references, click to expand

Identification and partial characterization of an inhibitor of collagenase from rabbit bone.
Biochem J. 1977 Nov 1;167(2):353-60 PMID: 202252
Evidence that species specificity and rate of collagen degradation are properties of collagen, not collagenase.
Biochim Biophys Acta. 1977 Sep 27;494(1):198-208 PMID: 198007
The detection and characterisation of collagenase inhibitors from rabbit tissues in culture.
Biochim Biophys Acta. 1977 Aug 11;483(2):493-8 PMID: 196653
Evidence that latent collagenases are enzyme-inhibitor complexes.
Biochem J. 1977 May 1;163(2):303-7 PMID: 194584
A new factor that may control collagen resorption.
Lancet. 1977 Aug 13;2(8033):333-5 PMID: 69939
Immunochemical studies with a specific antiserum to rabbit fibroblast collagenase.
Biochem J. 1975 Dec;151(3):655-63 PMID: 175786
Metalloproteases of human articular cartilage that digest cartilage proteoglycan at neutral and acid pH.
J Clin Invest. 1976 Oct;58(4):1030-41 PMID: 9425
An endopeptidase from rheumatoid synovial tissue culture.
Biochim Biophys Acta. 1972 Feb 28;258(2):566-76 PMID: 4334534
The interaction of alpha2-macroglobulin with proteinases. Binding and inhibition of mammalian collagenases and other metal proteinases.
Biochem J. 1974 May;139(2):359-68 PMID: 4374931
Stimulation by endocytosis of the secretion of collagenase and neutral proteinase from rabbit synovial fibroblasts.
J Exp Med. 1974 Dec 1;140(6):1482-97 PMID: 4372292
Proteoglycan-degrading enzymes. A radiochemical assay method and the detection of a new enzyme cathepsin F.
Biochem J. 1977 Dec 1;167(3):775-85 PMID: 23763

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1710493

Published

1978-05-01

Pages

493-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1183981

Subset

External Links

PubMed source DOI: 10.1042/bj1710493 PMC Full Text View journal details

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Neutral metallo-proteinases of rabbit bone. Separation in latent forms of distinct enzymes that when activated degrade collagen, gelatin and proteoglycans.

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