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PMID: 2072909 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Transcription factors nuclear factor I and Sp1 interact with the murine collagen alpha 1 (I) promoter.

Molecular and cellular biology ·Vol. 11 ·No. 8 ·1991-08-00 ·Pages 4065-73

Nehls MC, Rippe RA, Veloz L, Brenner DA

Abstract

The collagen alpha 1(I) promoter, which is efficiently transcribed in NIH 3T3 fibroblasts, contains four binding sites for trans-acting factors, as demonstrated by DNase I protection assays (D. A. Brenner, R. A. Rippe, and L. Veloz, Nucleic Acids Res. 17:6055-6064, 1989). This study characterizes the DNA-binding proteins that interact with the two proximal footprinted regions, both of which contain a reverse CCAAT box and a G + C-rich 12-bp direct repeat. Analysis by DNase I protection assays, mobility shift assays, competition with specific oligonucleotides, binding with recombinant proteins, and reactions with specific antisera showed that the transcriptional factors nuclear factor I (NF-I) and Sp1 bind to these two footprinted regions. Because of overlapping binding sites, NF-I binding and Sp1 binding appear to be mutually exclusive. Overexpression of NF-I in cotransfection experiments with the alpha 1(I) promoter in NIH 3T3 fibroblasts increased alpha 1(I) expression, while Sp1 overexpression reduced this effect, as well as basal promoter activity. The herpes simplex virus thymidine kinase promoter, which contains independent NF-I- and Sp1-binding sites, was stimulated by both factors. Therefore, expression of the collagen alpha 1(I) gene may depend on the relative activities of NF-I and Sp1.

MeSH Terms
Animals Base Sequence Binding Sites CCAAT-Enhancer-Binding Proteins Cell Line Cell Nucleus/physiology Collagen/genetics DNA-Binding Proteins/metabolism Deoxyribonuclease I Mice Molecular Sequence Data Mutagenesis, Site-Directed NFI Transcription Factors Nuclear Proteins/metabolism Oligonucleotide Probes Plasmids Promoter Regions, Genetic Protein Binding Sp1 Transcription Factor/metabolism Transcription Factors Transfection Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins NFI Transcription Factors Nuclear Proteins Oligonucleotide Probes Sp1 Transcription Factor Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human Collagen Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nehls M C
Department of Medicine, University of California, San Diego.
Rippe R A
Veloz L
Brenner D A
References (46)
46 references, click to expand
  1. Transforming growth factor beta increases mRNA for matrix proteins both in the presence and in the absence of changes in mRNA stability.
    Proc Natl Acad Sci U S A. 1988 Feb;85(4):1105-8 PMID: 3422482
  2. Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor.
    Cell. 1987 Jun 19;49(6):729-39 PMID: 3034432
  3. Human CCAAT-binding proteins have heterologous subunits.
    Cell. 1988 Apr 8;53(1):11-24 PMID: 3349524
  4. A high-efficiency HeLa cell nuclear transcription extract.
    DNA. 1988 Jan-Feb;7(1):47-55 PMID: 3349904
  5. A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs.
    Nature. 1988 Jul 21;334(6179):218-24 PMID: 3398920
  6. Type 1 plasminogen activator inhibitor gene: functional analysis and glucocorticoid regulation of its promoter.
    Proc Natl Acad Sci U S A. 1988 Aug;85(15):5525-9 PMID: 2840665
  7. Selective activation of transcription by a novel CCAAT binding factor.
    Science. 1988 Jul 29;241(4865):582-5 PMID: 3399893
  8. Sp1, a CAAT-binding factor, and the adenovirus major late promoter transcription factor interact with functional regions of the gamma-fibrinogen promoter.
    Mol Cell Biol. 1988 Jun;8(6):2628-37 PMID: 3043186
  9. Point mutations and small substitution mutations in three different upstream elements inhibit the activity of the mouse alpha 2(I) collagen promoter.
    J Biol Chem. 1988 Sep 25;263(27):13909-15 PMID: 3417682
  10. Sp1 transcription factor binds DNA and activates transcription even when the binding site is CpG methylated.
    Genes Dev. 1988 Sep;2(9):1127-35 PMID: 3056778
  11. Regulatory elements in the 5'-flanking region and the first intron contribute to transcriptional control of the mouse alpha 1 type I collagen gene.
    Mol Cell Biol. 1989 May;9(5):2224-7 PMID: 2747648
  12. Transcription factors AP-3 and AP-2 interact with the SV40 enhancer in a mutually exclusive manner.
    EMBO J. 1989 May;8(5):1455-60 PMID: 2548845
  13. Analysis of the collagen alpha 1(I) promoter.
    Nucleic Acids Res. 1989 Aug 11;17(15):6055-64 PMID: 2771638
  14. Jun-B differs in its biological properties from, and is a negative regulator of, c-Jun.
    Cell. 1989 Dec 22;59(6):979-86 PMID: 2513128
  15. An immunohistochemical study of architectural remodeling and connective tissue synthesis in pulmonary fibrosis.
    Am Rev Respir Dis. 1989 Dec;140(6):1693-703 PMID: 2604297
  16. Development of diffuse fasciitis with eosinophilia during L-tryptophan treatment: demonstration of elevated type I collagen gene expression in affected tissues. A clinicopathologic study of four patients.
    Ann Intern Med. 1990 Mar 1;112(5):344-51 PMID: 2306063
  17. Nuclear factor-I and activator protein-2 bind in a mutually exclusive way to overlapping promoter sequences and trans-activate the human growth hormone gene.
    Nucleic Acids Res. 1990 Jan 11;18(1):57-64 PMID: 2308836
  18. Human-mouse interspecies collagen I heterotrimer is functional during embryonic development of Mov13 mutant mouse embryos.
    Mol Cell Biol. 1990 Apr;10(4):1452-60 PMID: 1690840
  19. Two different negative and one positive regulatory factors interact with a short promoter segment of the alpha 1 (I) collagen gene.
    J Biol Chem. 1990 Jun 15;265(17):9934-42 PMID: 2351683
  20. SV40 stimulates expression of the transacting factor Sp1 at the mRNA level.
    Genes Dev. 1990 Apr;4(4):659-66 PMID: 2163346
  21. The B subunit of a rat heteromeric CCAAT-binding transcription factor shows a striking sequence identity with the yeast Hap2 transcription factor.
    Proc Natl Acad Sci U S A. 1990 Jul;87(14):5378-82 PMID: 2196566
  22. The human cytomegalovirus 2.7-kilobase RNA promoter contains a functional binding site for the adenovirus major late transcription factor.
    J Virol. 1990 Sep;64(9):4189-98 PMID: 2166813
  23. Co-localization of transforming growth factor beta 2 with alpha 1(I) procollagen mRNA in tissue sections of patients with systemic sclerosis.
    J Clin Invest. 1990 Sep;86(3):917-22 PMID: 1697606
  24. GC box binding induces phosphorylation of Sp1 by a DNA-dependent protein kinase.
    Cell. 1990 Oct 5;63(1):155-65 PMID: 2170018
  25. Contactpoint analysis of the HeLa nuclear factor I recognition site reveals symmetrical binding at one side of the DNA helix.
    EMBO J. 1987 Jan;6(1):161-8 PMID: 3034574
  26. High-efficiency transformation of mammalian cells by plasmid DNA.
    Mol Cell Biol. 1987 Aug;7(8):2745-52 PMID: 3670292
  27. The adenovirus major late transcription factor activates the rat gamma-fibrinogen promoter.
    Science. 1987 Oct 30;238(4827):684-8 PMID: 3672119
  28. Acetaldehyde increases collagen gene transcription in cultured human fibroblasts.
    J Biol Chem. 1987 Dec 25;262(36):17690-5 PMID: 3693368
  29. A simplified method for the preparation of transcriptionally active liver nuclear extracts.
    DNA Cell Biol. 1990 Dec;9(10):777-81 PMID: 2264931
  30. Retrovirus-induced interference with collagen I gene expression in Mov13 fibroblasts is maintained in the absence of DNA methylation.
    Mol Cell Biol. 1991 Jan;11(1):47-54 PMID: 1702514
  31. DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein.
    Cell. 1991 Mar 22;64(6):1123-34 PMID: 2004419
  32. Synergistic activation of a human promoter in vivo by transcription factor Sp1.
    Mol Cell Biol. 1991 Apr;11(4):1935-43 PMID: 2005889
  33. An upstream regulatory region mediates high-level, tissue-specific expression of the human alpha 1(I) collagen gene in transgenic mice.
    Mol Cell Biol. 1991 Apr;11(4):2066-74 PMID: 2005897
  34. Developmental expression of Sp1 in the mouse.
    Mol Cell Biol. 1991 Apr;11(4):2189-99 PMID: 2005904
  35. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  36. Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells.
    J Mol Appl Genet. 1983;2(1):101-9 PMID: 6302193
  37. The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
    Cell. 1983 Nov;35(1):79-87 PMID: 6313230
  38. Embryonic lethal mutation in mouse collagen I gene causes rupture of blood vessels and is associated with erythropoietic and mesenchymal cell death.
    Cell. 1984 Sep;38(2):597-607 PMID: 6467375
  39. Multiple specific contacts between a mammalian transcription factor and its cognate promoters.
    Nature. 1984 Nov 29-Dec 5;312(5993):409-13 PMID: 6095100
  40. Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro.
    Cell. 1985 Sep;42(2):559-72 PMID: 2992804
  41. Activation of the AIDS retrovirus promoter by the cellular transcription factor, Sp1.
    Science. 1986 May 9;232(4751):755-9 PMID: 3008338
  42. Ethanol-induced liver fibrosis in rats fed high fat diet.
    Hepatology. 1986 Sep-Oct;6(5):814-22 PMID: 3758935
  43. Transcriptional control of the mouse alpha 2(I) collagen gene: functional deletion analysis of the promoter and evidence for cell-specific expression.
    Mol Cell Biol. 1986 Feb;6(2):347-54 PMID: 3785151
  44. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.
    Cell. 1987 Jan 16;48(1):79-89 PMID: 3024847
  45. Firefly luciferase gene: structure and expression in mammalian cells.
    Mol Cell Biol. 1987 Feb;7(2):725-37 PMID: 3821727
  46. A nuclear factor 1 binding site mediates the transcriptional activation of a type I collagen promoter by transforming growth factor-beta.
    Cell. 1988 Feb 12;52(3):405-14 PMID: 2830985
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.8.4065-4073.1991
Published
1991-08-00
Pages
4065-73
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361214
Subset
IM
Grants
NIDDK NIH HHS · DK07202 · United States
NIDDK NIH HHS · DK38652 · United States
NIGMS NIH HHS · GM41804 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.8.4065-4073.1991 PMC Full Text View journal details
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