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PMID: 207555 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Chemical synthesis of a hexadecapeptide segment of ubiquitin that activates adenylate cyclase and induces lymphocytes to differentiate.

Experientia ·Vol. 34 ·No. 6 ·1978-06-15 ·Pages 703-4

Schlesinger DH, Goldstein G, Scheid MP, Bitensky M

Abstract

A hexadecapeptide corresponding to positions 59--74 of ubiquitin was synthesized and purified. The peptide was characterized by its mobility in TLC and electrophoresis, amino acid sequence and composition, and molar rotation. The peptide possessed approximately 40% activity compared with native ubiquitin in each of 3 biological assays in vitro: a) thymocyte induction, b) B cell induction and c) elevation of intracellular cyclic AMP levels in sarcoma 180 cells.

MeSH Terms

Adenylyl Cyclases/metabolism Amino Acid Sequence Animals B-Lymphocytes/drug effects Cell Differentiation/drug effects Cyclic AMP/metabolism Enzyme Activation Mice Nucleoproteins Peptides/chemical synthesis,pharmacology Sarcoma 180/metabolism

Chemicals

Nucleoproteins Peptides Cyclic AMP Adenylyl Cyclases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Schlesinger D H

Goldstein G

Scheid M P

Bitensky M

References (13)

13 references, click to expand

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Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells.
Proc Natl Acad Sci U S A. 1975 Jan;72(1):11-5 PMID: 1078892
Chemical synthesis of a peptide fragment of thymopoietin II that induces selective T cell differentiation.
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The complete amino acid sequence of ubiquitin, an adenylate cyclase stimulating polypeptide probably universal in living cells.
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The NH2- and COOH-terminal amino acid sequence of nuclear protein A24.
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In-vitro demonstration of thymic hormone in the mouse by conversion of precursor cells into lymphocytes.
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Isolation and chemical properties of two calcitonins from salmon ultimobranchial glands.
J Biol Chem. 1970 Mar 25;245(6):1491-6 PMID: 5442830
Molecular conservation of 74 amino acid sequence of ubiquitin between cattle and man.
Nature. 1975 May 29;255(5507):423-4 PMID: 1128706
Thymopoietin and myasthenia gravis: neostigmine-responsive neuromuscular block produced in mice by a synthetic peptide fragment of thymopoietin.
Lancet. 1975 Aug 9;2(7928):256-9 PMID: 49801
The amino acid sequence of thymopoietin II.
Cell. 1975 Aug;5(4):361-5 PMID: 1171728
A protein binding assay for adenosine 3':5'-cyclic monophosphate.
Proc Natl Acad Sci U S A. 1970 Sep;67(1):305-12 PMID: 4318781
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Article Info

Journal

Experientia

Abbr.

Experientia

ISSN

0014-4754

DOI

10.1007/BF01947269

Published

1978-06-15

Pages

703-4

Language

English

Region

Switzerland

NLM ID

0376547

Subset

External Links

PubMed source DOI: 10.1007/BF01947269

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Chemical synthesis of a hexadecapeptide segment of ubiquitin that activates adenylate cyclase and induces lymphocytes to differentiate.

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