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PMID: 20801887 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Vesicle fusion probability is determined by the specific interactions of munc18.

The Journal of biological chemistry ·Vol. 285 ·No. 49 ·2010-12-03 ·Pages 38141-8

Smyth AM, Rickman C, Duncan RR

Abstract

Mammalian-regulated secretion is absolutely dependent on four evolutionarily conserved proteins: three SNARE proteins and munc18. Dissecting the functional outcomes of the spatially organized protein interactions between these factors has been difficult because of the close interrelationship between different binding modes. Here, we investigated the spatial distribution of single munc18 molecules at the plasma membrane of cells and the underlying interactions between syntaxin and munc18. Disruption of munc18 binding to the N-terminal peptide motif of syntaxin did not alter munc18 localization on the plasma membrane but had a pronounced influence on the behavior of secretory vesicles and their likelihood to undergo fusion. We therefore conclude that interaction with the syntaxin N-peptide can confer differential release probabilities to secretory vesicles and may contribute to the delineation of secretory vesicle pools.

MeSH Terms
Amino Acid Motifs Animals Cell Membrane/genetics,metabolism Membrane Fusion/physiology Munc18 Proteins/genetics,metabolism PC12 Cells Qa-SNARE Proteins/genetics,metabolism Rats SNARE Proteins/genetics,metabolism Secretory Vesicles/genetics,metabolism
Chemicals
Munc18 Proteins Qa-SNARE Proteins SNARE Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Smyth Annya M
Centre for Integrative Physiology, University of Edinburgh, George Square, Edinburgh EH8 9XD, United Kingdom.
Rickman Colin
Duncan Rory R
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
DOI
10.1074/jbc.M110.164038
Published
2010-12-03
Epub
2010-00-26
Pages
38141-8
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC2992247
Subset
IM
Grants
Medical Research Council · United Kingdom
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1074/jbc.M110.164038 PMC Full Text View journal details
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