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PMID: 17205130 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Docking of secretory vesicles is syntaxin dependent.

PloS one ·Vol. 1 ·2006-12-27 ·Pages e126

de Wit H, Cornelisse LN, Toonen RF, Verhage M

Abstract

Secretory vesicles dock at the plasma membrane before they undergo fusion. Molecular docking mechanisms are poorly defined but believed to be independent of SNARE proteins. Here, we challenged this hypothesis by acute deletion of the target SNARE, syntaxin, in vertebrate neurons and neuroendocrine cells. Deletion resulted in fusion arrest in both systems. No docking defects were observed in synapses, in line with previous observations. However, a drastic reduction in morphologically docked secretory vesicles was observed in chromaffin cells. Syntaxin-deficient chromaffin cells showed a small reduction in total and plasma membrane staining for the docking factor Munc18-1, which appears insufficient to explain the drastic reduction in docking. The sub-membrane cortical actin network was unaffected by syntaxin deletion. These observations expose a docking role for syntaxin in the neuroendocrine system. Additional layers of regulation may have evolved to make syntaxin redundant for docking in highly specialized systems like synaptic active zones.

MeSH Terms
Animals Botulinum Toxins/genetics Cells, Cultured Chromaffin Cells/physiology,ultrastructure Gene Expression Gene Targeting Green Fluorescent Proteins/genetics Membrane Fusion/physiology Mice Microscopy, Electron, Transmission Munc18 Proteins/physiology Qa-SNARE Proteins/deficiency,genetics,physiology Secretory Vesicles/physiology,ultrastructure
Chemicals
Munc18 Proteins Qa-SNARE Proteins Stxbp1 protein, mouse enhanced green fluorescent protein Green Fluorescent Proteins Botulinum Toxins botulinum toxin type C
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
de Wit Heidi
Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam and VU Medical Center, Amsterdam, Netherlands.
Cornelisse L Niels
Toonen Ruud F G
Verhage Matthijs
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0000126
Published
2006-12-27
Epub
2006-00-27
Pages
e126
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC1762430
Subset
IM
Grants
HSRD VA · CDA 10-036 · United States
External Links
PubMed source DOI: 10.1371/journal.pone.0000126 PMC Full Text View journal details
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