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PMID: 8223426 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport.

The EMBO journal ·Vol. 12 ·No. 11 ·1993-11-00 ·Pages 4095-104

Aalto MK, Ronne H, Keränen S

Abstract

The yeast SEC1 gene encodes a hydrophilic protein that functions at the terminal stage in secretion. We have cloned two yeast genes, SSO1 and SSO2, which in high copy number can suppress sec1 mutations and also mutations in several other late acting SEC genes, such as SEC3, SEC5, SEC9 and SEC15. SSO1 and SSO2 encode small proteins with N-terminal hydrophilic domains and C-terminal hydrophobic tails. The two proteins are 72% identical in sequence and together perform an essential function late in secretion. Sso1p and Sso2p show significant sequence similarity to six other proteins. Two of these, Sed5p and Pep12p, are yeast proteins that function in transport from ER to Golgi and from Golgi to the vacuole, respectively. Also related to Sso1p and Sso2p are three mammalian proteins: epimorphin, syntaxin A/HPC-1 and syntaxin B. A nematode cDNA product also belongs to the new protein family. The new protein family is thus present in a wide variety of eukaryotic cells, where its members function at different stages in vesicular transport.

MeSH Terms
Amino Acid Sequence Antigens, Surface/genetics Base Sequence Biological Evolution Biological Transport Cell Compartmentation Cloning, Molecular Fungal Proteins/genetics GTP-Binding Proteins/genetics Genes, Fungal Genes, Suppressor Intracellular Membranes/metabolism,ultrastructure Membrane Proteins/genetics Models, Biological Models, Genetic Molecular Sequence Data Multigene Family/genetics Munc18 Proteins Nerve Tissue Proteins/genetics Qa-SNARE Proteins Saccharomyces cerevisiae/genetics,metabolism,ultrastructure Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Syntaxin 1 Vesicular Transport Proteins
Chemicals
Antigens, Surface Fungal Proteins Membrane Proteins Munc18 Proteins Nerve Tissue Proteins Qa-SNARE Proteins SEC1 protein, S cerevisiae SEC15 protein, S cerevisiae SSO1 protein, S cerevisiae SSO2 protein, S cerevisiae Saccharomyces cerevisiae Proteins Syntaxin 1 Vesicular Transport Proteins GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Aalto M K
VTT Biotechnical Laboratory, Espoo, Finland.
Ronne H
Keränen S
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-11-00
Pages
4095-104
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413702
Subset
IM
Databases
GENBANK
D10392, D10475, M75825, M90395, M95734, M95735, X66980, X67729, X67730
External Links
PubMed source PMC Full Text View journal details
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