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PMID: 1396561 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Bos1p, a membrane protein required for ER to Golgi transport in yeast, co-purifies with the carrier vesicles and with Bet1p and the ER membrane.

The EMBO journal ·Vol. 11 ·No. 10 ·1992-10-00 ·Pages 3609-17

Newman AP, Groesch ME, Ferro-Novick S

Abstract

BOS1 and BET1 are required for transport from the ER to the Golgi complex in yeast and genetically interact with each other and a subset of the other genes, whose products function at this stage of the secretory pathway. In a previous study, we reported that BOS1 encodes a putative 27 kDa membrane protein. Here we show that BET1 is structurally similar to the synaptobrevins and identical to the SLY12 gene product. Overexpression of SLY12 compensates for the loss of function of the ras-like GTP-binding protein Ypt1. Both Bos1p and Bet1p are cytoplasmically oriented membrane proteins. Bos1p co-purifies with the ER to Golgi transport vesicles and co-fractionates with Bet1p and the ER membrane.

Related Genes
BET1 BOS1 SLY12 ras
MeSH Terms
Base Sequence Carrier Proteins/genetics,isolation & purification,metabolism Centrifugation, Density Gradient DNA, Fungal/genetics,isolation & purification Endoplasmic Reticulum/metabolism Genes, Fungal Genes, Suppressor Golgi Apparatus/metabolism Membrane Proteins/genetics,isolation & purification,metabolism Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction Qb-SNARE Proteins RNA, Fungal/genetics,isolation & purification Recombinant Fusion Proteins/immunology,isolation & purification Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Vesicular Transport Proteins
Chemicals
BOS1 protein, S cerevisiae Carrier Proteins DNA, Fungal Membrane Proteins Oligodeoxyribonucleotides Qb-SNARE Proteins RNA, Fungal Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Vesicular Transport Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Newman A P
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510.
Groesch M E
Ferro-Novick S
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-10-00
Pages
3609-17
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556820
Subset
IM
Grants
NIGMS NIH HHS · 1 RO1 GM45431 · United States
NCI NIH HHS · CA 46128 · United States
External Links
PubMed source PMC Full Text View journal details
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