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PMID: 6282895 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Hepatic Golgi fractions resolved into membrane and content subfractions.

The Journal of cell biology ·Vol. 92 ·No. 3 ·1982-03-00 ·Pages 822-32

Howell KE, Palade GE

Abstract

Golgi fractions isolated from rat liver homogenates have been resolved into membrane and content subfractions by treatment with 100 mM Na2CO3 pH 11.3. This procedure permitted extensive extraction of content proteins and lipoproteins, presumably because it caused an alteration of Golgi membranes that minimized the reformation of closed vesicles. The type and degree of contamination of the fractions was assessed by electron microscopy and biochemical assays. The membrane subfraction retained 15% of content proteins and lipids, and these could not be removed by various washing procedures. The content subfraction was contaminated by both membrane fragments and vesicles and accounted for 5 to 10% of the membrane enzyme activities of the original Golgi fraction. The lipid compositions of the subfractions was determined, and the phospholipids of both membrane and content were found to be uniformly labeled with [33P]phosphate administered in vivo.

MeSH Terms
Animals Cell Fractionation Golgi Apparatus/analysis,ultrastructure Intracellular Membranes/analysis Lipids/analysis Liver/ultrastructure Male Phospholipids/analysis Phosphoric Monoester Hydrolases/metabolism Proteins/analysis Rats Rats, Inbred Strains
Chemicals
Lipids Phospholipids Proteins Phosphoric Monoester Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Howell K E
Palade G E
References (46)
46 references, click to expand
  1. Secretion granules of the rabbit parotid. Selective removal of secretory contaminants from granule membranes.
    J Cell Biol. 1978 Feb;76(2):323-40 PMID: 10605441
  2. Lipid transport in liver. I. Electron microscopic identification of very low density lipoproteins in perfused rat liver.
    Lab Invest. 1967 Feb;16(2):305-19 PMID: 6023786
  3. Intracellular transport of secretory proteins in the pancreatic exocrine cell. II. Transport to condensing vacuoles and zymogen granules.
    J Cell Biol. 1967 Aug;34(2):597-615 PMID: 6035648
  4. Lipid synthesis, intracellular transport, storage, and secretion. I. Electron microscopic radioautographic study of liver after injection of tritiated palmitate or glycerol in fasted and ethanol-treated rats.
    J Cell Biol. 1967 May;33(2):319-39 PMID: 6039375
  5. Lipid composition and turnover of rough and smooth microsomal membranes in rat liver.
    J Lipid Res. 1968 Nov;9(6):720-9 PMID: 5685264
  6. Regulation of production and release of lipoprotein by the perfused rat liver.
    J Lipid Res. 1968 Sep;9(5):613-9 PMID: 5726321
  7. Characterization of lipoprotein particles isolated from the Golgi apparatus of rat liver.
    J Lipid Res. 1969 Jul;10(4):433-9 PMID: 4978881
  8. Phospholipid class and fatty acid composition of golgi apparatus isolated from rat liver and comparison with other cell fractions.
    Biochemistry. 1970 Jan 6;9(1):19-25 PMID: 4312390
  9. Identity of very low density lipoprotein apoproteins of plasma and liver Golgi apparatus.
    Science. 1970 Apr 17;168(3929):380-2 PMID: 4985196
  10. Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots.
    Lipids. 1970 May;5(5):494-6 PMID: 5483450
  11. The biosynthesis of rat serum albumin. IV. Apparent passage of albumin through the Golgi apparatus during secretion.
    J Biol Chem. 1971 Jan 10;246(1):240-4 PMID: 5541766
  12. Radioautographic analysis of the secretory process in the parotid acinar cell of the rabbit.
    J Cell Biol. 1972 May;53(2):290-311 PMID: 5025103
  13. An improved scintillation cocktail of high-solubilizing power.
    Anal Biochem. 1973 Jan;51(1):173-9 PMID: 4688012
  14. Selective release of content from microsomal vesicles without membrane disassembly. I. Permeability changes induced by low detergent concentrations.
    J Cell Biol. 1973 Aug;58(2):436-62 PMID: 4729506
  15. Golgi fractions prepared from rat liver homogenates. I. Isolation procedure and morphological characterization.
    J Cell Biol. 1973 Oct;59(1):45-72 PMID: 4356571
  16. Golgi fractions prepared from rat liver homogenates. II. Biochemical characterization.
    J Cell Biol. 1973 Oct;59(1):73-88 PMID: 4356572
  17. Insulin binding to rat liver Golgi fractions.
    J Cell Biol. 1973 Dec;59(3):771-6 PMID: 4357462
  18. Cytochemistry of Golgi fractions prepared from rat liver.
    J Cell Biol. 1974 Jan;60(1):8-25 PMID: 4358430
  19. Dynamics of cytoplasmic membranes in guinea pig pancreatic acinar cells. I. Synthesis and turnover of membrane proteins.
    J Cell Biol. 1974 Apr;61(1):1-13 PMID: 4819305
  20. Selective release of content from microsomal vesicles without membrane disassembly. II. Electrophoretic and immunological characterization of microsomal subfractions.
    J Cell Biol. 1974 Jun;61(3):789-807 PMID: 4209578
  21. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  22. Isolation and characterization of Golgi apparatus and membranes from rat liver.
    Methods Enzymol. 1974;31:180-91 PMID: 4138142
  23. Secretion granules of the rabbit parotid gland. Isolation, subfractionation, and characterization of the membrane and content subfractions.
    J Cell Biol. 1975 Jan;64(1):182-210 PMID: 162790
  24. Selective solubilization of proteins from red blood cell membranes by protein perturbants.
    J Supramol Struct. 1973;1(3):220-32 PMID: 4804837
  25. Lipid composition of the Golgi apparatus of rat kidney and liver in comparison with other subcellular organelles.
    Biochim Biophys Acta. 1975 Mar 24;380(3):357-69 PMID: 1169965
  26. Colchicine inhibition of plasma protein release from rat hepatocytes.
    J Cell Biol. 1975 Jul;66(1):42-59 PMID: 1141379
  27. Intracellular aspects of the process of protein synthesis.
    Science. 1975 Aug 1;189(4200):347-58 PMID: 1096303
  28. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  29. The ultrastructure of rat plasma lipoproteins.
    J Ultrastruct Res. 1975 Nov;53(2):180-92 PMID: 172650
  30. Evidence for the translocation of 5'-nucleotidase across hepatic membranes in vivo.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):4013-7 PMID: 1060084
  31. Distribution of protein-bound sugar residues in microsomal subfractions and Golgi membranes.
    Biochim Biophys Acta. 1976 May 21;433(3):496-508 PMID: 1276191
  32. Synthesis in vitro of intrinsic membrane proteins by free, membrane-bound, and Golgi apparatus-associated polyribosomes from rat liver.
    J Biol Chem. 1976 Aug 25;251(16):5054-68 PMID: 956176
  33. Presence of adenylate cyclase activity in Golgi and other fractions from rat liver. I. Biochemical determination.
    J Cell Biol. 1976 Sep;70(3):660-70 PMID: 821954
  34. Presence of adenylate cyclase activity in Golgi and other fractions from rat liver. II. Cytochemical localization within Golgi and ER membranes.
    J Cell Biol. 1976 Sep;70(3):671-84 PMID: 956270
  35. The endoplasmic reticulum: a cytochemist's view (a review).
    Proc Natl Acad Sci U S A. 1976 Aug;73(8):2781-7 PMID: 183210
  36. The lipid composition of plasma membrane subfractions originating from the three major functional domains of the rat hepatocyte cell surface.
    Biochim Biophys Acta. 1976 Dec 14;455(3):655-64 PMID: 999933
  37. Solubilization and properties of galactosyltransferase and sulfotransferase activities of Golgi membranes in Triton X-100.
    J Biol Chem. 1978 Mar 10;253(5):1632-8 PMID: 627561
  38. Cell fractionation studies on the guinea pig pancreas. Redistribution of exocrine proteins during tissue homogenization.
    J Cell Biol. 1978 Jul;78(1):110-30 PMID: 670290
  39. Endoplasmic reticulum marker enzymes in Golgi fractions--what does this mean?
    J Cell Biol. 1978 Nov;79(2 Pt 1):581-9 PMID: 214450
  40. Presence of NADPH-cytochrome P-450 reductase in rat liver Golgi membranes. Evidence obtained by immunoadsorption method.
    J Cell Biol. 1978 Nov;79(2 Pt 1):590-7 PMID: 214451
  41. Distribution of terminal glycosyltransferases in hepatic Golgi fractions.
    J Cell Biol. 1980 Jan;84(1):87-101 PMID: 7350172
  42. Heterogeneity of lipoprotein particles in hepatic Golgi fractions.
    J Cell Biol. 1982 Mar;92(3):833-45 PMID: 7085757
  43. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  44. A simple method for the isolation and purification of total lipides from animal tissues.
    J Biol Chem. 1957 May;226(1):497-509 PMID: 13428781
  45. A FAST, SIMPLE AND RELIABLE METHOD FOR THE MICRODETERMINATION OF PHOSPHORUS IN BIOLOGICAL MATERIALS.
    Anal Biochem. 1964 Feb;7:218-24 PMID: 14134436
  46. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid.
    J Biol Chem. 1960 Mar;235:769-75 PMID: 13793161
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.92.3.822
Published
1982-03-00
Pages
822-32
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112045
Subset
IM
Grants
NIGMS NIH HHS · GM 21714 · United States
NIGMS NIH HHS · GM 27303 · United States
External Links
PubMed source DOI: 10.1083/jcb.92.3.822 PMC Full Text View journal details
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