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PMID: 2195039 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of a functional vesicular intermediate that mediates ER to Golgi transport in yeast.

The Journal of cell biology ·Vol. 111 ·No. 1 ·1990-07-00 ·Pages 45-53

Groesch ME, Ruohola H, Bacon R, Rossi G, Ferro-Novick S

Abstract

We have used an in vitro assay that reconstitutes transport from the ER to the Golgi complex in yeast to identify a functional vesicular intermediate in transit to the Golgi apparatus. Permeabilized yeast cells, which serve as the donor in this assay, release a homogeneous population of vesicles that are biochemically distinct from the donor ER fraction. The isolated vesicles, containing a post-ER/pre-Golgi form of the marker protein pro-alpha-factor, were able to bind to and fuse with exogenously added Golgi membranes. The ability to isolate fusion competent vesicles provides direct evidence that ER to Golgi membrane transport is mediated by a discrete population of vesicular carriers.

MeSH Terms
Biological Transport Cytosol/metabolism,ultrastructure Endoplasmic Reticulum/metabolism,ultrastructure Golgi Apparatus/metabolism,ultrastructure Kinetics Mating Factor Models, Biological Molecular Weight Organelles/metabolism,ultrastructure Peptides/genetics,isolation & purification,metabolism Pheromones/genetics Protein Precursors/metabolism Saccharomyces cerevisiae/metabolism,ultrastructure
Chemicals
Peptides Pheromones Protein Precursors Mating Factor
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Groesch M E
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510-8002.
Ruohola H
Bacon R
Rossi G
Ferro-Novick S
References (31)
31 references, click to expand
  1. Protein glycosylation in yeast.
    Annu Rev Biochem. 1987;56:915-44 PMID: 3304149
  2. Purification and characterization of constitutive secretory vesicles from yeast.
    J Cell Biol. 1987 Jul;105(1):163-74 PMID: 3301865
  3. Transport of secretory and membrane glycoproteins from the rough endoplasmic reticulum to the Golgi. A rate-limiting step in protein maturation and secretion.
    J Biol Chem. 1988 Feb 15;263(5):2107-10 PMID: 3276683
  4. Temperature-sensitive steps in the transport of secretory proteins through the Golgi complex in exocrine pancreatic cells.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6425-9 PMID: 3462704
  5. Sequential intermediates in the pathway of intercompartmental transport in a cell-free system.
    Cell. 1984 Dec;39(3 Pt 2):525-36 PMID: 6096009
  6. Intracellular transport of secretory proteins in the pancreatic exocrine cell. IV. Metabolic requirements.
    J Cell Biol. 1968 Dec;39(3):589-603 PMID: 5699933
  7. Pre- and post-Golgi vacuoles operate in the transport of Semliki Forest virus membrane glycoproteins to the cell surface.
    Cell. 1984 Sep;38(2):535-49 PMID: 6432345
  8. The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery.
    Cell. 1988 Mar 25;52(6):915-24 PMID: 3127057
  9. Intracellular transport of secretory proteins in the pancreatic exocrine cell. I. Role of the peripheral elements of the Golgi complex.
    J Cell Biol. 1967 Aug;34(2):577-96 PMID: 6035647
  10. A study of the immunochemistry of three yeast mannans.
    J Biol Chem. 1970 Mar 10;245(5):1197-203 PMID: 5461381
  11. Increased amounts of HMG-CoA reductase induce "karmellae": a proliferation of stacked membrane pairs surrounding the yeast nucleus.
    J Cell Biol. 1988 Jul;107(1):101-14 PMID: 3292536
  12. Study of a temperature-sensitive mutant of the ras-related YPT1 gene product in yeast suggests a role in the regulation of intracellular calcium.
    Cell. 1988 May 20;53(4):635-47 PMID: 3286011
  13. Reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex in yeast: the acceptor Golgi compartment is defective in the sec23 mutant.
    J Cell Biol. 1988 Oct;107(4):1465-76 PMID: 3049622
  14. Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack.
    Cell. 1989 Jul 28;58(2):329-36 PMID: 2752426
  15. Temperature and energy dependence of secretory protein transport in the exocrine pancreas.
    EMBO J. 1986 Jul;5(7):1477-82 PMID: 3743549
  16. The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants.
    J Cell Biol. 1989 Sep;109(3):1015-22 PMID: 2504726
  17. Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis.
    J Cell Biol. 1986 May;102(5):1543-50 PMID: 3517001
  18. Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-alpha-factor.
    Cell. 1984 Jul;37(3):1075-89 PMID: 6430565
  19. Order of events in the yeast secretory pathway.
    Cell. 1981 Aug;25(2):461-9 PMID: 7026045
  20. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts.
    J Bacteriol. 1946 Sep;52(3):293-301 PMID: 16561177
  21. A novel prefusion complex formed during protein transport between Golgi cisternae in a cell-free system.
    J Biol Chem. 1986 Feb 15;261(5):2202-7 PMID: 3003101
  22. Enzymes required for yeast prohormone processing.
    Annu Rev Physiol. 1988;50:345-62 PMID: 3288097
  23. A vesicular intermediate in the transport of hepatoma secretory proteins from the rough endoplasmic reticulum to the Golgi complex.
    J Cell Biol. 1987 Feb;104(2):221-30 PMID: 3027103
  24. Sec53, a protein required for an early step in secretory protein processing and transport in yeast, interacts with the cytoplasmic surface of the endoplasmic reticulum.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8468-72 PMID: 3317409
  25. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  26. Isolation of secretory vesicles from Saccharomyces cerevisiae.
    Anal Biochem. 1987 Nov 1;166(2):328-34 PMID: 3324821
  27. In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent posttranslational translocation of the prepro-alpha-factor.
    Cell. 1986 May 9;45(3):397-406 PMID: 3009026
  28. Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection.
    J Cell Biol. 1987 Oct;105(4):1587-94 PMID: 3312234
  29. Intracellular aspects of the process of protein synthesis.
    Science. 1975 Aug 1;189(4200):347-58 PMID: 1096303
  30. Isolation of a vesicular intermediate in the cell-free transfer of membrane from transitional elements of the endoplasmic reticulum to Golgi apparatus cisternae of rat liver.
    J Biol Chem. 1988 Nov 25;263(33):17738-48 PMID: 3182869
  31. Isolation of exocytic carrier vesicles from BHK cells.
    Cell. 1989 Aug 25;58(4):719-27 PMID: 2504496
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.1.45
Published
1990-07-00
Pages
45-53
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116157
Subset
IM
Grants
NCI NIH HHS · CA46128 · United States
External Links
PubMed source DOI: 10.1083/jcb.111.1.45 PMC Full Text View journal details
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