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PMID: 3292536 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Increased amounts of HMG-CoA reductase induce "karmellae": a proliferation of stacked membrane pairs surrounding the yeast nucleus.

The Journal of cell biology ·Vol. 107 ·No. 1 ·1988-07-00 ·Pages 101-14

Wright R, Basson M, D'Ari L, Rine J

Abstract

Overproduction of the enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in yeast resulted in striking morphological effects on the structure of intracellular membranes. Specifically, stacks of paired membranes closely associated with the nuclear envelope were observed in strains that over-produced the HMG1 isozyme, one of two isozymes for HMG-CoA reductase in yeast. These nuclear-associated, paired membranes have been named "karmellae." In strains that overproduced the HMG1 isozyme, HMG-CoA reductase was present in the karmellar layers. At mitosis, karmellae were asymmetrically segregated: the mother cells inherited all of the karmellae and the daughter cells inherited none. A membranous structure of different morphology was occasionally found in cells that overproduced the HMG2 isozyme. These observations further establish the existence of cellular mechanisms that monitor the levels of membrane proteins and compensate for changes in these levels by inducing synthesis of particular types of membrane.

MeSH Terms
Cell Nucleus/ultrastructure Hydroxymethylglutaryl CoA Reductases/biosynthesis Immunohistochemistry Intracellular Membranes/enzymology,ultrastructure Isoenzymes/biosynthesis Microscopy, Electron Mitosis Nuclear Envelope/enzymology,ultrastructure Saccharomyces cerevisiae/cytology,enzymology,ultrastructure
Chemicals
Isoenzymes Hydroxymethylglutaryl CoA Reductases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wright R
Department of Biochemistry, University of California, Berkeley 94720.
Basson M
D'Ari L
Rine J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.1.101
Published
1988-07-00
Pages
101-14
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115167
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.107.1.101 PMC Full Text View journal details
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