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PMID: 20810283 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Review

DNA methylation and cellular reprogramming.

Trends in cell biology ·Vol. 20 ·No. 10 ·2010-10-00 ·Pages 609-17

De Carvalho DD, You JS, Jones PA

Abstract

The recent discovery that a small number of defined factors are sufficient to reprogram somatic cells into pluripotent stem cells has significantly expanded our knowledge of the plasticity of the epigenome. In this review we discuss some aspects of cell fate plasticity and epigenetic alterations, with emphasis on DNA methylation during cellular reprogramming. Recent data suggest that DNA methylation is a major barrier to induced pluripotent stem (iPS) cell reprogramming. The demethylating agent 5-azacytidine can enhance the efficiency of iPS cells generation and the putative DNA demethylase protein activation-induced cytidine deaminase (AID/AICDA) can erase DNA methylation at pluripotency gene promoters, thereby allowing cellular reprogramming. Elucidation of the epigenetic changes taking place during cellular reprogramming will enhance our understanding of stem cell biology and facilitate therapeutic applications.

MeSH Terms
Animals Cell Differentiation Cellular Reprogramming DNA Methylation Epigenesis, Genetic Humans Transcription Factors/metabolism
Chemicals
Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
De Carvalho Daniel D
Department of Urology, University of Southern California Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
You Jueng Soo
Jones Peter A
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Article Info
Journal
Trends in cell biology
Abbr.
Trends Cell Biol
ISSN
1879-3088
DOI
10.1016/j.tcb.2010.08.003
Published
2010-10-00
Epub
2010-00-31
Pages
609-17
Language
English
Region
England
NLM ID
9200566
PMCID
PMC2981432
Subset
IM
Grants
NCI NIH HHS · R01 CA083867 · United States
NCI NIH HHS · R37 CA082422 · United States
NCI NIH HHS · R01 CA 83867 · United States
NCI NIH HHS · R37 CA 082422 · United States
External Links
PubMed source DOI: 10.1016/j.tcb.2010.08.003 PMC Full Text View journal details
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