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PMID: 20876112 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Disruption of the Arabidopsis CGI-58 homologue produces Chanarin-Dorfman-like lipid droplet accumulation in plants.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 107 ·No. 41 ·2010-10-12 ·Pages 17833-8

James CN, Horn PJ, Case CR, Gidda SK, Zhang D, Mullen RT, Dyer JM, Anderson RG, Chapman KD

Abstract

CGI-58 is the defective gene in the human neutral lipid storage disease called Chanarin-Dorfman syndrome. This disorder causes intracellular lipid droplets to accumulate in nonadipose tissues, such as skin and blood cells. Here, disruption of the homologous CGI-58 gene in Arabidopsis thaliana resulted in the accumulation of neutral lipid droplets in mature leaves. Mass spectroscopy of isolated lipid droplets from cgi-58 loss-of-function mutants showed they contain triacylglycerols with common leaf-specific fatty acids. Leaves of mature cgi-58 plants exhibited a marked increase in absolute triacylglycerol levels, more than 10-fold higher than in wild-type plants. Lipid levels in the oil-storing seeds of cgi-58 loss-of-function plants were unchanged, and unlike mutations in β-oxidation, the cgi-58 seeds germinated and grew normally, requiring no rescue with sucrose. We conclude that the participation of CGI-58 in neutral lipid homeostasis of nonfat-storing tissues is similar, although not identical, between plant and animal species. This unique insight may have implications for designing a new generation of technologies that enhance the neutral lipid content and composition of crop plants.

MeSH Terms
1-Acylglycerol-3-Phosphate O-Acyltransferase/genetics,metabolism Age Factors Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics,metabolism DNA Primers/genetics Fatty Acids/analysis Humans Lipids/chemistry Mass Spectrometry Microscopy, Fluorescence Plant Leaves/metabolism Reverse Transcriptase Polymerase Chain Reaction Seeds/chemistry Triglycerides/analysis
Chemicals
Arabidopsis Proteins DNA Primers Fatty Acids Lipids Triglycerides 1-Acylglycerol-3-Phosphate O-Acyltransferase ABHD5 protein, human
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
James Christopher N
Department of Biological Sciences, Center for Plant Lipid Research, University of North Texas, Denton, TX 76203, USA.
Horn Patrick J
Case Charlene R
Gidda Satinder K
Zhang Daiyuan
Mullen Robert T
Dyer John M
Anderson Richard G W
Chapman Kent D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0911359107
Published
2010-10-12
Epub
2010-00-27
Pages
17833-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2955100
Subset
IM
Grants
NHLBI NIH HHS · P01 HL020948 · United States
NIGMS NIH HHS · R01 GM052016 · United States
NIGMS NIH HHS · GM-52016 · United States
NHLBI NIH HHS · HL-20948 · United States
External Links
PubMed source DOI: 10.1073/pnas.0911359107 PMC Full Text View journal details
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