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PMID: 20878143 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mapping 49 quantitative trait loci at high resolution through sequencing-based genotyping of rice recombinant inbred lines.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 122 ·No. 2 ·2011-02-00 ·Pages 327-40

Wang L, Wang A, Huang X, Zhao Q, Dong G, Qian Q, Sang T, Han B

Abstract

Mapping chromosome regions responsible for quantitative phenotypic variation in recombinant populations provides an effective means to characterize the genetic basis of complex traits. We conducted a quantitative trait loci (QTL) analysis of 150 rice recombinant inbred lines (RILs) derived from a cross between two cultivars, Oryza sativa ssp. indica cv. 93-11 and Oryza sativa ssp. japonica cv. Nipponbare. The RILs were genotyped through next-generation sequencing, which accurately determined the recombination breakpoints and provided a new type of genetic markers, recombination bins, for QTL analysis. We detected 49 QTL with phenotypic effect ranging from 3.2 to 46.0% for 14 agronomics traits. Five QTL of relatively large effect (14.6-46.0%) were located on small genomic regions, where strong candidate genes were found. The analysis using sequencing-based genotyping thus offers a powerful solution to map QTL with high resolution. Moreover, the RILs developed in this study serve as an excellent system for mapping and studying genetic basis of agricultural and biological traits of rice.

MeSH Terms
China Chromosome Mapping/methods Crops, Agricultural/genetics Crosses, Genetic Genes, Plant Genetic Markers Inflorescence/genetics Oryza/genetics Phenotype Plant Shoots/genetics Quantitative Trait Loci Recombination, Genetic Seeds/genetics
Chemicals
Genetic Markers
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Wang Lu
National Center for Gene Research and Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai, 200233, China.
Wang Ahong
Huang Xuehui
Zhao Qiang
Dong Guojun
Qian Qian
Sang Tao
Han Bin
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Article Info
Journal
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Abbr.
Theor Appl Genet
ISSN
1432-2242
DOI
10.1007/s00122-010-1449-8
Published
2011-02-00
Epub
2010-00-28
Pages
327-40
Language
English
Region
Germany
NLM ID
0145600
PMCID
PMC3021254
Subset
IM
External Links
PubMed source DOI: 10.1007/s00122-010-1449-8 PMC Full Text View journal details
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